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A novel stimulator of protein phosphorylation in Neurospora crassa

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Summary

An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin “in vitro” phosphorylation.

The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed.

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Abbreviations

PK:

protein kinase

MES:

2-N-Morpholino ethane-sulfonic acid

PMSF:

phenylmethylsulfonyl fluoride

S100 :

100000 × g Supernatant

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Authors and Affiliations

  1. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI) CONICET, Obligado 2490, 1428, Buenos Aires, Argentina

    Rita M. Ulloa, Gerardo C. Glikin, María T. Téllez-Ĩnón, Héctor N. Torres & Norberto D. Judewicz

  2. Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428, Buenos Aires, Argentina

    Rita M. Ulloa, Gerardo C. Glikin, María T. Téllez-Ĩnón, Héctor N. Torres & Norberto D. Judewicz

Authors
  1. Rita M. Ulloa
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  2. Gerardo C. Glikin
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  3. María T. Téllez-Ĩnón
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  4. Héctor N. Torres
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  5. Norberto D. Judewicz
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Ulloa, R.M., Glikin, G.C., Téllez-Ĩnón, M.T. et al. A novel stimulator of protein phosphorylation in Neurospora crassa . Mol Cell Biochem 77, 11–17 (1987). https://doi.org/10.1007/BF00230146

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  • DOI: https://doi.org/10.1007/BF00230146

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