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Effect of calmodulin inhibitor, Stelazine, on the endocytosis of vitellogenin and transglutaminase activity in Xenopus laevis Oöcytes

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Summary

The specific endocytosis of vitellogenin was measured in the presence of various concentrations of Stelazine, a specific inhibitor of the calcium regulating protein, calmodulin. Stelazine (200 µ M) was found to inhibit the endocytosis of vitellogenin by 63% as determined by decreased uptake of vitellogenin into transitional yolk bodies and yolk platelets. The enzymatic activity of transglutaminase, a calcium-requiring enzyme implicated in receptor-mediated endocytosis, was not affected by these concentrations of Stelazine.

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Authors and Affiliations

  1. Dept. of Cell and Molecular Biology, Medical College of Georgia, 30912, Augusta, GA, USA

    L. M. Tucciarone & K. D. Lanclos

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  1. L. M. Tucciarone
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  2. K. D. Lanclos
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Tucciarone, L.M., Lanclos, K.D. Effect of calmodulin inhibitor, Stelazine, on the endocytosis of vitellogenin and transglutaminase activity in Xenopus laevis Oöcytes. Mol Cell Biochem 45, 159–161 (1982). https://doi.org/10.1007/BF00230084

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  • DOI: https://doi.org/10.1007/BF00230084

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