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Purification and characterization of allergens from Xanthium strumarium pollen

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Abstract

The allergenic components present in whole pollen extract of Xanthium strumarium were isolated by sequential ammonium sulphate precipitation, DEAE Sephadex A50 chromatography and gel filtration. The techniques of RAST inhibition and skin test were utilized to check the allergenicity of fractionated proteins revealing the presence of Xan Ib and Xan VIa as the important allergenic componenets. Xan Ib was found to be devoid of carbohydrate and had a molecular weight of 103 000 daltons. Xan VIa was a glycoprotein of molecular weight 17 000 daltons. The carbohydrate moiety of Xan Vla was found to be associated with allergenicity. The characteristic pattern of whole pollen extract on CIE and TLIEF showed 36 and 21 protein bands, respectively. The use of FPLC in isolation of partially purified allergens from Xanthium is discussed.

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  1. Kuldeep S. Jaggi

    Present address: Department of Immunology, University of Manitoba, 730 William Avenua, R3E OW3, Winnipeg, MB, Canada

Authors and Affiliations

  1. CSIR Centre for Biochemicals, G.T.B. Marg, Delhi University Campus, 110007, Delhi, India

    Kuldeep S. Jaggi & Sharad V. Gangal

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  1. Kuldeep S. Jaggi
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  2. Sharad V. Gangal
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Jaggi, K.S., Gangal, S.V. Purification and characterization of allergens from Xanthium strumarium pollen. Mol Cell Biochem 78, 177–190 (1987). https://doi.org/10.1007/BF00229692

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  • DOI: https://doi.org/10.1007/BF00229692

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