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Characterization of DNA binding proteins released from sarcoma-180 chromatin during brief digestion with DNase-I

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Summary

Brief digestion of sarcoma-180 chromatin (≤ 5%) by pancreatic DNase-I releases six non-histone proteins with Mol. wt. 21.5K, 26K, 72.5K, 77K, 90K and 120K dalton and pI values ranging from 4.7 to 12.4. The protein of Mol. wt. 77K dalton was obtained at high alkaline range of pH = 12.4. The antibodies against these proteins induce dose dependent inhibition in transcription of native chromatin. Results suggest a role of these proteins in positive control of gene transcription in sarcoma-180 cells.

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Author notes

  1. Rajat K. Neogy

    Present address: Department of Pharmacy, Jadavpur University, 700032, Calcutta, India

Authors and Affiliations

  1. Department of Biochemistry, Chittaranjan National Cancer Research Centre, 37, S.P. Mookherjee Road, 700026, Calcutta, India

    Chinmoy Kumar Panda, Kanakendu Choudhury & Rajat K. Neogy

Authors
  1. Chinmoy Kumar Panda
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  2. Kanakendu Choudhury
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  3. Rajat K. Neogy
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Panda, C.K., Choudhury, K. & Neogy, R.K. Characterization of DNA binding proteins released from sarcoma-180 chromatin during brief digestion with DNase-I. Mol Cell Biochem 78, 101–108 (1987). https://doi.org/10.1007/BF00229684

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  • DOI: https://doi.org/10.1007/BF00229684

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