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Role of oxidized human plasma low density lipoproteins in atherosclerosis: effects on smooth muscle cell proliferation

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Abstract

The effects of oxidized human plasma low density lipoproteins (Ox-LDL) on the proliferation of cultured aortic smooth muscle cells was studied, employing viable cell counting, [3H] thymidine incorporation into DNA, and the release of lactate dehydrogenase (LDH) into the medium. Oxidized LDL (prepared by incubation of LDL with copper sulfate) exerted a concentration-dependent stimulation (2 fold, compared to control) of aortic smooth muscle cell proliferation at low concentrations (0.1 µg – 10 µg/ml medium). On the other hand, at high concentrations (25–200 µg/ml), Ox-LDL produced a pronounced decrease in viable cells, a decrease in the incorporation of [3H] thymidine into DNA, and an increase in the release of LDH in the medium. In this report, the previously postulated biological roles of oxidized-LDL in atherosclerosis are discussed in view of these findings.

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Abbreviations

Ox-LDL:

Oxidized human plasma Low Density Lipoproteins

SMC:

Smooth Muscle Cells

LDH:

Lactate Dehydrogenase

LPC:

Lysophosphatidycholine

PC:

Phosphatidylcholine

TNF:

Tumor Necrosis Factor

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  1. Lipid Research, Atherosclerosis Unit, Department of Pediatrics, Johns Hopkins University, School of Medicine, 21205, Baltimore, Maryland, USA

    Subroto Chatterjee

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  1. Subroto Chatterjee
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Chatterjee, S. Role of oxidized human plasma low density lipoproteins in atherosclerosis: effects on smooth muscle cell proliferation. Mol Cell Biochem 111, 143–147 (1992). https://doi.org/10.1007/BF00229586

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