Skip to main content
SpringerLink
Log in

Lipid peroxidation during myocardial reperfusion

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Reperfusion of heart muscle after prolonged ischaemia is associated with metabolic and functional abnormalities and eventual cell death. Free radical induced lipid peroxidation of cell membranes is thought to be a major mechanism in the evolution of reperfusion damage. The evidences in support for this kind of damage are based on tissue malondialdehyde quantitation by the thiobarbituric acid test (TBA-test). In an attempt to verify this topic we have subjected isolated and Langendorff perfused rabbit hearts to a period of 60 minutes of severe ischaemia plus 30 minutes of reperfusion. At appropriate time points malondialdehyde was determined in the tissue by means of TBA-test and directly by reversed phase, high pressure, liquid chromatography (HPLC).

We have found no correlation between the two compared assays. During reperfusion, there was the formation of non-lipid related, malondialdehyde-like, TBA-reactive substance which leads to overstimations of the extent of lipid peroxidation.

On the contrary, by direct HPLC quantitation, there was a decrease of tissue malondialdehyde during ischaemia and during the early phases of reperfusion. Our results demonstrate that TBA-test is not a reliable index of malondialdehyde accumulation in organ system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bolli R: Oxygen-derived free radicals and post-ischaemic myocardial disfunction (Stunned Myocardium). J Am Coll Cardiol 12: 239–249, 1988

    Google Scholar 

  2. Beuge JA, Aust SD: Microsomal lipid peroxidation. In: S Fleisher, L Packer, (eds.) Methods in Enzymology: Biomembranes. New York, London, Paris. Academic Press, Vol 52: 302–310, 1978

    Google Scholar 

  3. Bull AW, Marnett LJ: Determination of malondialdehyde by ion-pairing high-performance liquid chromatography. Anal Biochem 149: 284–290, 1985

    Google Scholar 

  4. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 246–254, 1976

    Google Scholar 

  5. Burton KP: Evidence of direct toxic effects of free radicals on the myocardium. Free Rad Biol Med 4: 15–24, 1988

    Google Scholar 

  6. Ceconi C, Curello S, Cargnoni A, Ferrari R, Albertini A, Visioli O: The role of glutathione status in the protection against ischaemic and reperfusion damage: effects of N-acetyl cysteine. J Mol Cell Cardiol 20: 5–13, 1988

    Google Scholar 

  7. Chambers DJ, Astras G, Takahashi A, Manning AS, Braimbridge MV, Hearse DJ: Free radicals and cardioplegia: organic anti-oxidants as additives to the St Thomas' Hospital cardioplegic solution Cardiovasc Res 23: 351–358, 1989

    Google Scholar 

  8. Curello S, Ceconi C, Bigoli C, Ferrari R, Albertini A, Guarnieri C: Changes in the cardiac glutathione status after ischaemia and reperfusion. Experientia 41: 42–43, 1985

    Google Scholar 

  9. Esterbauer H, Lang J, Zadravec S, Slater F: Detection of malondialdehyde by high-performance liquid chromatography. In: L Packer (ed.) Methods in Enzymology: Oxygen Radicals in Biological Systems. New York, London, Paris. Academic Press, 105: 319, 1984

    Google Scholar 

  10. Ferrari R, Ceconi C, Curello S, Guarnieri C, Caldarera CM, Albertini A, Visioli O: Oxygen-mediated myocardial damage during ischaemia and reperfusion: role of the cellular defences against oxygen toxicity. J Mol Cell Cardiol 17: 937–945, 1985

    Google Scholar 

  11. Ferrari R, Ceconi C, Curello S, Cargnoni A, Agnoletti G, Boffa GM, Visioli O: Intracellular effects of myocardial ischaemia and reperfusion: role of calcium and oxygen. Eur Heart J 7: 3–12, 1986

    Google Scholar 

  12. Garlick PB, Davies MJ, Hearse DJ, Slater TF: Direct detection of free radicals in the reperfused rat heart using electron spin resonance spectroscopy. Circ Res 5: 757–760, 1987

    Google Scholar 

  13. Guarnieri C, Flamigni F, Caldarera CM: Role of oxygen in the cellular damage induced by re-oxygenation of hypoxic heart. J Mol Cell Cardiol 12: 7987–808, 1980

    Google Scholar 

  14. Gutteridge JMC, Stocks TL, Dormandy TL: Thiobarbituric acid reacting substances derived from autooxidizing linoleic and linolenic acids. Anal Acta 70(1): 107–111, 1974

    Google Scholar 

  15. Gutteridge JMC: Thiobarbituric acid-reactivity following iron-dependent free-radical damage to amino acids and carbohydrates. FEBS Letters 128: 343–346, 1981

    Google Scholar 

  16. Halliwel B: Oxidants and human disease: some new concepts. FASEB J 1: 358–364, 1087

    Google Scholar 

  17. Janero DR, Burghardt B: Analysis of cardiac membrane phospholipid peroxidation kinetics as malondialdehyde: non specificity of thiobarbituric acid-reactivity. Lipids 23: 452–458, 1988

    Google Scholar 

  18. Jolly SR, Kane WJ, Bailie MB, Abrams GD, Lucchesi BR: Canine myocardial reperfusion injury; its reduction by the combined administration of superoxide dismutase and catalase. Circ Res 54: 277–285, 1984

    Google Scholar 

  19. Julicher RHM, Tijburg LBM, Sterrenberg L, Bast A, Koomen JR, Noordhoek J: Decreased defence against free radicals in rat heart during normal reperfusion after hypoxic, ischaemic and calcium-free perfusion. Life Sci 35: 1281–1288, 1984

    Google Scholar 

  20. Kukuda Y, Stanley DW, Van De Voort FR: Determination of TBA humber by high performance liquid chromatography. J Am Oil Chem Soc 58: 773–775, 1981

    Google Scholar 

  21. Liedtke AJ, Mahar CO, Ytrehus K, Mjos OD: Estimates of free radical production in rat and swine hearts: method and application of measuring malondialdehyde levels in fresh and frozen myocardium. Basic Res Cardiol 79: 513–518, 1984

    Google Scholar 

  22. Marx JL: Oxygen free radicals linked to many diseases. Science 235: 529–531, 1987

    Google Scholar 

  23. Meerson FZ, Kagan VE, Kozov YuP, Belkina LM, Arkhipenko YuV: The role of lipid peroxidation in pathogenesis of ischaemia damage and the antioxidant protection of the heart. Bas Res Card 77: 163–185, 1982

    Google Scholar 

  24. Mitsos SE, Fantone JC, Gallagher KP, Walden KM, Simpson PJ, Abrams GD, Schork MA, Lucchesi BR: Canine myocardial reperfusion injury: protection by a free radical scavenger, N-2-mercaptopropionyl glycine. J Cardiovasc Pharmacol 8: 978–988, 1986

    Google Scholar 

  25. Ohkawa H, Ohishi N, Yagi K: Reaction of linoleic acid hydroperoxide with thiobarbituric acid. J Lipid Res 19: 1053–1057, 1978

    Google Scholar 

  26. Ohkawa H, Ohishi N, Yagi K: Assay for lipid peroxides in animal tissues by Thiobarbituric Acid Reaction. Anal Biochem 95: 351–358, 1979

    Google Scholar 

  27. Oliver TA: A spectrophotometric method for the determination of creatine phosphokinase and myokinase. Biochem J 61: 116–122, 1955

    Google Scholar 

  28. Otani H, Umemoto M, Kagawa K, Nakamura Y, Omoto K, Tanaka K, Sato T, Nonoyama A, Kagawa T: Protection against oxygen-induced reperfusion injury of the isolated canine heart by superoxide dismutase and catalase. J Surg Res 41: 126–133, 1986

    Google Scholar 

  29. Rao PS, Cohen MV, Mueller HA: Production of free radicals and peroxides in early experimental myocardial ischaemia. J Mol Cell Cardiol 12: 797–808, 1980

    Google Scholar 

  30. Simpson PJ, Lucchesi BR: Free radicals and myocardial ischaemia and reperfusion injury. J Lab Clin Med 110(1): 13–30, 1987

    Google Scholar 

  31. Slater TF: Free Radical Mechanism in Tissue Injury. London: J.R. Legnoto (ed.) Pion, 38, 1972

  32. Stewart JR, Crute SL, Loughlin V, Hess HL, Greenfield LJ: Prevention of free radical-induced myocardial reperfusion injury with allopurinol. J Thorac Cardiovasc Surg 90: 68–72, 1985

    Google Scholar 

  33. Subramanian R, Plehn S, Noonan J, Schmidt M, Shug AL: Free radical-mediated damage during myocardial ischaemia and reperfusion and protection by carnitine esters. Z Kardiol 76: 41–45, 1987

    Google Scholar 

  34. Warren L: The thiobarbituric acid assay of sialic acid. J Biol Chem 234: 1971–1975, 1959

    Google Scholar 

  35. Torok B, Roth E, Bar V, Pollak Z: Effects of antioxidant therapy in experimentally induced heart infarcts. Basic Res Cardiol 81: 167–179, 1986

    Google Scholar 

  36. Zweier JL: Measurement of superoxide-derived free radicals in the reperfused heart. Evidence for a free radical mechanism of reperfusion injury. J Biol Chem 263: 1353–1357, 1988

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cattedra di Cardiologia dell ‘Universita’ degli Studi di Brescia, P.le Spedali Civili, 1, 25100, Brescia, Italy

    C. Ceconi, A. Cargnoni, E. Pasini, E. Condorelli, S. Curello & R. Ferrari

Authors
  1. C. Ceconi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Cargnoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Pasini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Condorelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. Curello
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. R. Ferrari
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ceconi, C., Cargnoni, A., Pasini, E. et al. Lipid peroxidation during myocardial reperfusion. Mol Cell Biochem 111, 49–54 (1992). https://doi.org/10.1007/BF00229573

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00229573

Key words

Search

Navigation