Theoretical and Applied Genetics

, Volume 84, Issue 3–4, pp 397–402

Characterization of species-specific repeated DNA sequences from B. nigra

  • Vibha Gupta
  • G. LakshmiSita
  • M. S. Shaila
  • V. Jagannathan
  • Malathi S. Lakshmikumaran
Article

DOI: 10.1007/BF00229499

Cite this article as:
Gupta, V., LakshmiSita, G., Shaila, M.S. et al. Theoret. Appl. Genetics (1992) 84: 397. doi:10.1007/BF00229499

Summary

The construction and characterization of two genome-specific recombinant DNA clones from B. nigra are described. Southern analysis showed that the two clones belong to a dispersed repeat family. They differ from each other in their length, distribution and sequence, though the average GC content is nearly the same (45%). These B genome-specific repeats have been used to analyse the phylogenetic relationships between cultivated and wild species of the family Brassicaceae.

Key words

Repeated DNA Brassica species Genome specificity Phylogenetic studies 

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Vibha Gupta
    • 1
  • G. LakshmiSita
    • 2
  • M. S. Shaila
    • 2
  • V. Jagannathan
    • 1
  • Malathi S. Lakshmikumaran
    • 1
  1. 1.Tata Energy Research InstituteNew DelhiIndia
  2. 2.Microbiology and Cell Biology DepartmentIndian Institute of ScienceBangaloreIndia

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