Theoretical and Applied Genetics

, Volume 84, Issue 3–4, pp 397–402 | Cite as

Characterization of species-specific repeated DNA sequences from B. nigra

  • Vibha Gupta
  • G. LakshmiSita
  • M. S. Shaila
  • V. Jagannathan
  • Malathi S. Lakshmikumaran
Article

Summary

The construction and characterization of two genome-specific recombinant DNA clones from B. nigra are described. Southern analysis showed that the two clones belong to a dispersed repeat family. They differ from each other in their length, distribution and sequence, though the average GC content is nearly the same (45%). These B genome-specific repeats have been used to analyse the phylogenetic relationships between cultivated and wild species of the family Brassicaceae.

Key words

Repeated DNA Brassica species Genome specificity Phylogenetic studies 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Vibha Gupta
    • 1
  • G. LakshmiSita
    • 2
  • M. S. Shaila
    • 2
  • V. Jagannathan
    • 1
  • Malathi S. Lakshmikumaran
    • 1
  1. 1.Tata Energy Research InstituteNew DelhiIndia
  2. 2.Microbiology and Cell Biology DepartmentIndian Institute of ScienceBangaloreIndia

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