Summary
To identify the mildew resistance locus Mla in barley with molecular markers, closely linked genomic RFLP clones were selected with the help of near-isogenic lines having the ‘Pallas’ and ‘Siri’ background. Out of 22 polymorphic clones 3 were located around the Mla locus on chromosome 5 with a distance of 5.1 + 2.9 cM (MWG 1H068), 4.2±1.7 cM (MWG 1H060) and 0.7 ± 0.7 cM (MWG 1H036), respectively. The polymorphic clone MWG 1H036 displayed the same RFLP pattern in both ‘Pallas’ and ‘Siri’ near-isogenic lines and in different varieties digested with six restriction enzymes possessing the same mildew resistance gene. The alleles of the Mla locus were grouped in 11 classes according to their specific RFLP patterns; 3 of these groups contain the majority of Mla alleles already used in barley breeding programs in Europe.
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Aslam M, Schwarzbach E (1980) An inoculation technique for quantitative studies of brown rust resistance in barley. Phytopath Z 99:87–91
Barone A, Ritter E, Schachtschabel U, Debner T, Salamini F, Gebhardt C (1990) Localisation by restriction fragment length polymorphism mapping in potato of a major dominant gene conferring resistance to the potato cyst nematode Globodera rostochiensis. Mol Gen Genet 224:177–182
Bernatzky R, Tanksley SD (1986) Majority of random cDNA clones correspond to single loci in the tomato genome. Mol Gen Genet 203:8–14
Bonierbale MW, Plaisted RL, Tanksley SD (1988) RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103
Burr B, Burr FA, Thompson KH, Albertson MC, Stuber CW (1988) Gene mapping with recombinant inbreeds in maize. Genetics 118:519–526
Fischbeck G (1991) Barley cultivar development in Europe success in the past and possible changes in the future. In: Proc VI Int Barley Genet Symp. Helsingborg, Barley Genetics VI, (in press)
Flor HH (1955) Host-parasite interaction in flax rust — its genetics and other implication. Phytopathology 45:680–685
Gebhardt C, Ritter E, Debner T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage mapping in Solonun tuberosum. Theor Appl Genet 78:65–75
Giese H (1981) Powdery mildew resistance genes in the Ml-a and Ml-k regions on barley chromosome 5. Hereditas 95:51–62
Graner A, Siedler H, Jahoor A, Herrmann RG, Wenzel G (1990) Assessment of the degree and the type of restriction fragment length polymorphism in barley (Hordeum vulgare). Theor Appl Genet 80:826–832
Graner A, Jahoor A, Schondelmaier J, Siedler H, Pillen K, Fischbeck G, Wenzel G, Herrmann RG (1991) Construction of an RFLP map of barley. Theor Appl Genet 83:250–256
Helentjaris T (1987) A genetic linkage map for maize baised on RFLPs. Trends Genet 3:217–221
Heun M, Kennedy AE, Anderson JA, Lapitan NLV, Sorrells ME, Tanksley SD (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437–447
Hinze K, Thompson RD, Ritter E, Salamini F, Schulze-Lefert P (1991) RFLP-mediated targeting of the ml-o resistance locus in barley (Hordeum vulgare) Proc Natl Acad Sci USA 88:3691–3695
Hiura U (1960) Studies on the disease resistance in barley. IV. Genetics of resistance to powdery mildew. Ber Ohara Inst Landwirtsch Biol Okayama Univ 11:235–300
Islam AKMR, Shepherd KW (1981) Wheat-barley addition lines: their use in genetic and evolutionary studies of barley. In: Proc IV Int Barley Genet Symp. Edinburgh, Barley Genetics IV, pp 729–39
am MR, Jahoor A, Fischbeck G (1991) Reactions of differential barley lines carrying Mla alleles to European and Israeli powdery mildew isolates. In: Proc VI Int Barley Genet Symp. Helsingborg, (ed. L. Munk) Barley Genetics IV, pp. 589–92
Jahoor A (1987) Mehltauresistenz israelischer Wildgersten — Resistenzspektrum, Vererbung und Lokalisierung. Ph.D. thesis, Technische Universität München
Jahoor A, Fischbeck G (1987) Source of resistance to powdery mildew in barley lines derived from Hordeum spontaneum collected in Israel. Plant Breed 99:274–281
Jahoor A, Backes G, Graner A, Herrmann RG, Fischbeck G (1991 a) Development of RFLP markers for the barley genome. Plant Breed 107:73–76
Jahoor A, Schondelmaier J, Graner A, Herrmann RG, Fischbeck G (1991b) Linkage analysis of RFLP markers in the barley genome. In: Proc VI Int Barley Genet Symp. Helsingborg, (ed. L. Munk) Barley Genetics VI, pp 263–65
Jung C, Klein M, Fischer F, Herrmann RG (1990) Analysis of DNA from a Beta procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance. Theor Appl Genet 79:663–672
Klein-Lankhorst R, Rietvelt P, Machiels B, Verkerk R, Weide R, Gebhardt C, Koornneef M, Zabel P (1991) RFLP markers linked to the root knot nematode resistance gene Mi in tomato Theor Appl Genet 81:661–667
Kølster P, Munk L, Stølen O, Lohde J (1986) Near-isogenic barley lines with genes for resistance to powdery mildew. Crop Sci 26:903–907
Kølster P, Stølen O (1987) Barley isolines with genes for resistance to Erysiphe graminis f. sp. hordei in the recurrent parent ‘Siri’. Plant Breed 98:79–82
Landry BS, Kesseli RV, Farrara B, Michelmore RW (1987) A genetic map of lettuce (Lactuca sativa L.) with restriction fragment length polymorphism, isozyme disease resistance and morphological markers. Genetics 116:331–337
Limpert E, Andrivon D, Fischbeck G (1990) Virulence patterns in populations of Erysiphe graminisf. sp. hordei in Europe in 1986. Plant Pathol 39:402–415
Manners JM, Davidson AD, Scott KJ (1985) Patterns of non-infectional protein synthesis in barley carrying different genes for resistance to powdery mildew. Plant Mol Biol 4:275–283
Messeguer R, Ganal M, de Vicente MC, Young ND, Bolkan H, Tanksley SD (1991) High resolution RFLP map around the root knot nematode resistance gene Mi in tomato. Theor Appl Genet 82:529–536
Michalek W (1991) Einsatz von RFLP Markern zur Lokalisierung von Donorsegmenten in den nahisogenen Linien für Mehltauresistenz bei Gerste. M. Sc. thesis, Technische Universität München
Moseman JG (1959) Host-pathogen interaction of the genes for resistance in Hordeum vulgare and for pathogenicity in Erysiphe graminis f. sp. hordei. Phytopathology 49:469–472
Orkin SH (1986) Reverse genetics and human disease. Cell 47:845–850
Sarfatti M, Katan J, Fluhr R, Zamir D (1989) An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene I2. Theor Appl Genet 78:755–759
Siedler H, Graner A (1991) Construction of physical maps of the Hor1 locus of two barley cultivars by pulsed field gel electrophoresis. Mol Gen Genet 226:177–181
Sørensen MB (1989) Mapping of the Hor2 locus in barley by pulsed field gel electrophoresis. Carlsberg Res Commun 54:109–120
Weiss H (1991) Vergleich des Polymorphiegrades zwischen Wildgerste und Kulturgerste mit dem innerhalb von Kulturgersten sowie Versuche zur Identifizierung von Transposons in Gerstenmutanten. M. Sc. thesis, Technische Universität München
Worton RG, Thompson MW (1988) Genetic of Duchenne muscular dystrophy. Annu Rev Genet 22:601–629
Young ND, Zamir D, Ganal MW, Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the Tm-2a gene in tomato. Genetics 120:579–587
Yu ZH, Mackill DJ, Bonman JM, Tanksley SD (1991) Tagging genes for blast resistance in rice via linkage to RFLP markers. Theor Appl Genet 81:471–476
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Communicated by G. Wenzel
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Schüller, C., Backes, G., Fischbeck, G. et al. RFLP markers to identify the alleles on the Mla locus conferring powdery mildew resistance in barley. Theoret. Appl. Genetics 84, 330–338 (1992). https://doi.org/10.1007/BF00229491
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DOI: https://doi.org/10.1007/BF00229491