Summary
Mitochondrial DNA (mtDNA) was isolated from young green leaves of rice plants. DNA fragments were cloned into lambda DNA, and clones that hybridized to mitochondrial genes from other plants were selected. Distal restriction fragments of these clones were used as probes for the selection of overlapping clones. A genetic map was finally created from the library by “walking” along the genome. The mitochondrial genome consists of five basic circles, with each circle sharing homologous sequences with one or two other circles. A master circle was constructed from the results of recombination across repeated sequences, and its size was estimated to be 492 kb. A physical map and a bank of overlapping clones were also constructed.
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References
Fauron CM-R, Havlik M, Brettell RIS (1990) The mitochondrial genome organization of a maize fertile cmsT revertant line is generated through recombination between two sets of repeats. Genetics 124:423–428
Folkerts O, Hanson MR (1989) Three copies of a single recombination repeat occur on the 443-kb mastercircle of the Petunia hybrida 3704 mitochondrial genome. Nucleic Acids Res 17:7345–7357
Gualberto JM, Wintz H, Weil JH, Grienenberger JM (1988) The genes coding for subunit 3 of NADH dehydrogenase and for ribosomal protein S12 are present in the wheat and maize mitochondrial genes and are co-transcribed. Mol Gen Genet 215:118–127
Kadowaki K, Ishige T, Suzuki S, Harada K, Shinjyo C (1986) Differences in the characteristics of mitochondrial DNA between normal and male sterile cytoplasms of Japonica rice. J pn J Breed 36:333–339
Lonsdale DM, Hodge TP, Fauron CM-R, Flavell RB (1983) A predicted structure for mitochondrial genome from the fertile cytoplasm of maize. UCLA Symp Mol Cell Biol New Ser 12:445–456
Lonsdale DM, Hodge TP, Fauron CM-R (1984) The physical map and organization of the mitochondrial genome from the fertile cytoplasm of maize. Nucleic Acids Res 12:9249–9261
Newton KJ (1988) Plant mitochondrial genomes: organization, expression and variation. Annu Rev Plant Physiol Plant Mol Biol 39:503–532
Palmer JD (1985) Comparative organization of chloroplast genomes. Annu Rev Genet 19:325–354
Palmer JD, Shields CR (1984) Tripartite structure of the Brassica campestris mitochondrial genome. Nature 307:437–440
Pring DR, Lonsdale DM (1985) Molecular biology of higher plant mitochondrial DNA. Int Rev Cytol 97:1–46
Quetier F, Lejeune B, Delorme S, Falconet D, Jubier MF (1985) Molecular form and function of the wheat mitochondrial genome. In: van Volten-Doting L, Groot GSP, Hall TC (ededs) NATO ASI Ser. Life Sci: 83:413–420
Suzuki T, Kazama S, Hirai A, Akihama T, Kadowaki K (1991) The rice mitochondria nad3 gene has an extended reading frame in its 5′ end. Curr Genet 20:331–337
Umbeck PF, Gengenbach BG (1983) Reversion of male-sterile T-cytoplasms of maize to male fertility in tissue culture. Crop Sci 23:584–588
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Communicated by G. S. Khush
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Iwahashi, M., Nakazono, M., Kanno, A. et al. Genetic and physical maps and a clone bank of mitochondrial DNA from rice. Theoret. Appl. Genetics 84, 275–279 (1992). https://doi.org/10.1007/BF00229482
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DOI: https://doi.org/10.1007/BF00229482