Abstract
Autonomous division of the endosperm was induced by in vitro culture of unpollinated ovaries or placenta-attached ovules in Helleborus niger, Lupinus luteus and Melandrium album. The induction frequencies for the three species were 50%, 10–20% and 0.1%, respectively. The endosperms contained up to 20 free nuclei; only a few ovules with 80–420 endosperm nuclei were found. Induction of autonomous division of the endosperm, which is unusual in amphimictic plants, was observed in three new species. No embryos appeared in the ovules. This suggests a developmental independence of the endosperm from the embryo in the culture of unpollinated ovaries or ovules.
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References
Beasley CA (1977) Ovule culture: fundamental and pragmatic research for the cotton industry. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 160–178
Brouland M (1968) Recherches cytologiques sur la fécondation chez quelques renonculacées: Caltha palustris L., Anemone nemorosa L., Ranunculus acris L., Helleborus foetidus L. Rev Cytol Biol Veget 32:309–319
Chu CC (1978) The N6 medium and its applications to anther culture of cereal crops. In: Chinese Acad Sci (ed) Proceedings of the Symposium on Plant Tissue Culture. Science Press, Peking, pp 43–50
Clapham D (1971) In vitro development of callus from the pollen of Lolium and Hordeum. Z Pflanzenzucht 65:285–292
Czapik R (1991) Some research problems of the endosperm in Angiospermae. Pol Bot Stud 2:109–120
Gillaspy G, Ben-David H, Gruissem W (1993) Fruits: a developmental perspective. Plant Cell 5:1439–1451
Herr JM (1971) A new clearing-squash technique for the study of ovule development in angiosperms. Am J Bot 58:785–790
Hu SY, Li LG, Zhou C (1985) Isolation of viable embryo sacs and their protoplasts of Nicotiana tabacum. Acta Bot Sin 27:337–344
Huang BQ, Russel SD (1989) Isolation of fixed and viable eggs, central cells and embryo sacs from ovules of Plumbago zeylandica. Plant Physiol 90:9–12
Huang QF, Yang HY, Zhou C (1982) Embryological observations on ovary culture of unpollinated flowers in Hordeum vulgare L. Acta Bot Sin 24:295–300
Jensen WA (1962) Botanical histochemistry. Freeman, San Francisco
Jensen WA, Schulz P, Ashton ME (1977) An ultrastructural study of early endosperm development and synergid changes in unfertilized cotton ovules. Planta 133:179–189
Koltunow AM (1993) Apomixis: embryo sacs and embryos formed without meiosis or fertilization in ovules. Plant Cell 5:1425–1437
Kranz E, Bautor J, Lörz H (1991) Electrofusion-mediated transmission of cytoplasmic organelles through the in vitro fertilization process, fusion of sperm cells with synergids and central cells, and cell reconstitution in maize. Sex Plant Reprod 4:17–21
Laikova LI (1976) Cytoembryological findings about autonomous formation of endosperm in maize. In: Khokhlov SS (ed) Apomixis and breeding. Amerind, New Delhi, pp 109–112
Miller CO (1963) Kinetin and kinetin-like compounds. In: Linskens HF, Tracey MV (eds) Moderne Methoden der Pflanzen- analyse, vol 6. Springer, Berlin Heidelberg, pp 194–202
Mól R (1986) Isolation of protoplasts from female gametophytes of Torenia fournieri. Plant Cell Rep 3:202–206
Mól R (1992) In vitro gynogenesis in Melandrium album: from parthenogenetic embryos to mixoploid plants. Plant Sci 81:261–269
Mól R (1993) Embryo sac development during the culture of placenta attached ovules of Melandrium album. Biol Plant 35:25–30
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Tadeo FR, Talon M, Germain E, Dosba F (1994) Embryo sac development and endogenous gibberellins in pollinated and unpollinated ovaries of walnut (Juglans regia). Physiol Plant 91:37–44
Tian HQ, Yang HY (1983) Synergid apogamy and egg cell anomalous division in cultured ovaries of Oryza sativa L. Acta Bot Sin 25:403–408
Trela Z (1963a) Embryological studies in Anemone nemorosa L. Acta Biol Cracov 6:1–14
Trela Z (1963b) Cytological studies in the differentiation of the endosperm in Anemone nemorosa L. Acta Biol Cracov 6:177–183
Trela-Sawicka Z (1974) Embryological studies in Anemone ranunculoides L. from Poland. Acta Biol Cracov 17:1–25
Van Went JL, Kwee HS (1990) Enzymatic isolation of living embryo sac of Petunia. Sex Plant Reprod 3:257–262
Wagner VT, Song YC, Matthys-Rochon E, Dumas C (1989) Observations on the isolated embryo sac of Zea mays. Plant Sci 59:127–132
Wu Y, Zhou C, Koop HU (1993) Enzymatic isolation of viable nucelli at the megaspore mother cell stage and in developing embryo sacs in Nicotiana tabacum. Sex Plant Reprod 6:171–175
Yang HY, Zhou C (1982) In vitro induction of haploid plants from unpollinated ovaries and ovules. Theor Appl Genet 63:97–104
Zhou C, Yang HY (1981) Induction of haploid rice plantlets by ovary culture. Plant Sci Lett 20:231–237
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Mól, R., Betka, A. & Wojciechowicz, M. Induction of autonomous endosperm in Lupinus luteus, Helleborus niger and Melandrium album by in vitro culture of unpollinated ovaries. Sexual Plant Reprod 8, 273–277 (1995). https://doi.org/10.1007/BF00229383
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DOI: https://doi.org/10.1007/BF00229383