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Coupling of histone mRNA levels to radioresistant DNA synthesis in ataxia-telangiectasia cells

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Abstract

Cloned genomic DNA for human histone H1, H3 and H4 genes has been used to determine the effects of γ-radiation on histone mRNA levels and synthesis in ataxia-telangiectasia cells. Synthesis of histone mRNA was determined in cells synchronized with aphidicolin. Effects of irradiation on DNA synthesis and passage through S phase were also monitored. Irradiation was found to slow the passage of control cells through the cell cycle but had no effect on progression of ataxia-telangiectasia cells. H1 and core histone mRNA synthesis was inhibited by radiation in two control cell lines after release from aphidicolin block. No inhibition was observed in one ataxia-telangiectasia cell line and a small degree of inhibition in a second. An increased level of mRNA was observed in both irradiated control and ataxia-telangiectasia cells at 5–7 h post-irradiation compared to unirradiated cells. Similar results were obtained in log phase cells. These results demonstrate that histone mRNA synthesis is radioresistant in ataxia-telangiectasia cells and is coupled to radioresistant DNA synthesis in these cells.

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Authors and Affiliations

  1. Department of Biochemistry, University of Queensland, 4067, Brisbane, Australia

    Martin F. Lavin & Sharad Kumar

  2. Division of Biology, California Institute of Technology, Pasadena, CA, USA

    Jane Houldsworth

  3. Department of Microbiology, University of Florida College of Medicine, 32610, Gainsville, FL, USA

    Janet L. Stein

  4. Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, 32610, Gainsville, FL, USA

    Gary S. Stein

Authors
  1. Martin F. Lavin
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  2. Jane Houldsworth
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  3. Sharad Kumar
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  4. Janet L. Stein
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  5. Gary S. Stein
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Lavin, M.F., Houldsworth, J., Kumar, S. et al. Coupling of histone mRNA levels to radioresistant DNA synthesis in ataxia-telangiectasia cells. Mol Cell Biochem 73, 45–54 (1987). https://doi.org/10.1007/BF00229375

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  • DOI: https://doi.org/10.1007/BF00229375

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