Summary
Three corn (Zea mays L.) lines resistant to imidazolinone herbicides were developed by in vitro selection and plant regeneration. For all three lines, resistance is inherited as a single semidominant allele. The resistance alleles from resistant lines XA17, XI12, and QJ22 have been crossed into the inbred line B73, and in each case homozygotes are tolerant of commercial use rates of imidazolinone herbicides. All resistant selections have herbicide-resistant forms of acetohydroxyacid synthase (AHAS), the known site of action of imidazolinone herbicides. The herbicide-resistant phenotypes displayed at the whole plant level correlate directly with herbicide insensitivity of the AHAS activities of the selections. The AHAS activities from all three selections have normal feedback regulation by valine and leucine, and plants containing the mutations display a normal phenotype.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson PA, Georgeson M (1989) Herbicide-tolerant mutants of corn. Genome 31:994–999
Beyer EM Jr, Duffy MJ, Hay JV, Schlueter DD (1988) Sulfonylurea herbicides. In: Kearney PC, Kaufmann DD (eds) Herbicides: chemistry, degradation, and mode of action. Marcel Dekker, New York, pp 117–189
Botterman J, Leemans J (1988) Engineering herbicide resistance in plants. Trends Genet 4:219–222
Chaleff RS (1983) Isolation of agronomically useful mutants from plant cell cultures. Science 219:676–682
Chaleff RS, Mauvais CJ (1984) Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science 224:1443–1445
Chaleff RS, Parsons MF (1978) Direct selection in vitro for herbicide-resistant mutants of Nicotiana tabacum. Proc Natl Acad Sci USA 75:5104–5107
Chaleff RS, Ray TB (1984) Herbicide-resistant mutants from tobacco cell cultures. Science 223:1148–1151
Creason GL, Chaleff RS (1988) A second mutation enhances resistance of a tobacco mutant to sulfonylurea herbicides. Theor Appl Genet 76:177–182
Evans DA, Sharp WR (1986) Applications of somaclonal variation. Bio/technology 4:528–532
Fincher RR (1989) Transfer of in vitro selected imidazolinone resistance to commercial maize hybrids. In: Copping LG, Rodgers P (eds) Prospects for amino acid biosynthesis inhibitors in crop protection and pharmaceutical chemistry. British Crop Protection Council, Farnham, England, pp 69–73
Flick CE (1983) Isolation of mutants from cell culture. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture. John Wiley & Sons, New York, pp 393–441
Gasser CS, Fraley RT (1989) Genetically engineering plants for crop improvement. Science 244:1293–1299
Haughn GW, Somerville C (1986) Sulfonylurea-resistant mutants of Arabidopsis thaliana. Mol Gen Genet 204:430–434
Haughn GW, Somerville CR (1990) A mutation causing imidazolinone resistance maps to the Csr1 locus of Arabidopsis thaliana. Plant Physiol 92:1081–1085
Mazur BJ, Falco SC (1989) The development of herbicide resistant crops. Annu Rev Plant Physiol Plant Mol Biol 40:441–470
Miflin BJ (1971) Cooperative feedback control of barley acetohydroxyacid synthase by leucine, isoleucine, and valine. Arch Biochem Biophys 146:542–550
Miflin BJ, Cave PR (1972) The control of leucine, isoleucine, and valine biosynthesis in a range of higher plants. J Exp Bot 23:511–516
Miller OK, Hughes KW (1980) Selection of paraquat-resistant variants of tobacco from cell cultures. In Vitro 16:1085–1091
Newhouse KE, Shaner DL, Wang T, Fincher R (1990) Genetic modification of crop responses to imidazolinone herbicides. In: Green MB, LeBaron HM, Moberg WK (eds) Managing resistance to agrochemicals. American Chemical Society, Washington, pp 474–481
Rathinasabapathi B, Williams D, King J (1990) Altered feedback sensitivity to valine, leucine, and isoleucine of acetolactate synthase from herbicide resistant variants of Datura innoxia. Plant Sci 67:1–6
Relton JM, Wallsgrove RM, Bourgin JP, Bright SWJ (1986) Altered feedback sensitivity of acetohydroxyacid synthase from valine-resistant mutants of tobacco (Nicotiana tabacum). Planta 169:46–50
Saxena PK, King J (1990) Lack of cross-resistance of imidazolinone-resistant cell lines of Datura innoxia P. Mill. to chlorsulfuron. Plant Physiol 94:1111–1115
Sebastian SA, Fader GM, Ulrich JF, Forney DR, Chaleff RS (1989) Semidominant soybean mutation for resistance to sulfonylurea herbicides. Crop Sci 29:1403–1408
Shaner DL, Robson PA (1985) Absorption, translocation, and metabolism of AC 252,214 in soybean (Glycine max), common cocklebur (Xanthium strumarium), and velvetleaf (Abutilon theophrasti). Weed Sci 33:469–471
Shaner DL, Anderson PC, Stidham MA (1984) Imidazolinones: potent inhibitors of acetohydroxyacid synthase. Plant Physiol 76:545–546
Singh BK, Stidham MA, Shaner DL (1988) Assay of acetohydroxyacid synthase. Anal Biochem 171:173–179
Subramanian MV, Hung HY, Dias JM, Miner VW, Butler JH, Jachetta JJ (1990) Properties of mutant acetolactate synthases resistant to triazolopyrimidine sulfonanilide. Plant Physiol 94:239–244
Swanson EB, Herrgesell MJ, Arnoldo M, Sippell DW, Wong RSC (1989) Microspore mutagenesis and selection: Canola plants with field tolerance to the imidazolinones. Theor Appl Genet 78:525–530
Author information
Authors and Affiliations
Additional information
Communicated by J. W. Snape
Rights and permissions
About this article
Cite this article
Newhouse, K., Singh, B., Shaner, D. et al. Mutations in corn (Zea mays L.) conferring resistance to imidazolinone herbicides. Theoret. Appl. Genetics 83, 65–70 (1991). https://doi.org/10.1007/BF00229227
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00229227