Summary
The formation of stacks of smooth-surfaced cisternae was studied in samples of Tetrahymena pyriformis that were prepared for electron microscopy after starvation for 2 days in buffer, and in samples of organisms of different mating type that were starved for 2 days and then mixed to induce conjugation. The number of stacks of cisternae was greater in starved ciliates than in those from stock cultures, and the size and number of stacks increased further after mixing animals of different mating type. When cells were either starved or mixed in buffer to which the protein synthesis inhibitor cycloheximide had been added, the formation of the membranous stacks was almost completely abolished. Addition of the RNA synthesis inhibitor actinomycin D, however, did not result in a significant decrease in the size and number of the stacks of saccules. Conjugation did not occur in the presence of either cycloheximide or actinomycin. The results suggest that protein synthesis is required for the formation of the stacks, but RNA synthesis is no longer necessary for their formation at this stage. The previous identification of the stacks as a Golgi apparatus and the possible functions of these membranes are considered.
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Beams, H. W., Kessel, R. G.: The Golgi apparatus: Structure and function. Int. Rev. Cytol. 23, 209–276 (1968)
Brandes, D., Buetow, D. E., Bertini, F., Malkoff, D. B.: Role of lysosomes in cellular lytic processes. I. Effect of carbon starvation in Euglena gracilis. Exp. Mol. Path. 3, 583–609 (1964)
Dryl, S.: Antigenic transformation in Paramecium aurelia after homologous antiserum treatment during autogamy and conjugation. J. Protozool., Suppl. 6, 25 (1959)
Elliot, A. M., Kennedy, J. R.: Morphology of Tetrahymena. In: Biology of Tetrahymena: (ed. A. M. Elliott), p. 57–87. Stroudsburg, Pa.: Dowden Hutchinson and Ross 1973
Elliott, A. M., Tremor, J. W.: The fine structure of the pellicle in the contact area of conjugating Tetrahymena pyriformis. J. biophys. biochem. Cytol. 4, 839–840 (1958)
Elliott, A. M., Zieg, R. G.: A Golgi apparatus associated with mating in Tetrahymena pyriformis. J. Cell Biol. 36, 391–398 (1968)
Flickinger, C. J.: The development of Golgi complexes and their dependence upon the nucleus in amebae. J. Cell Biol. 43, 250–262 (1969)
Flickinger, C. J.: Decreased formation of Golgi bodies in amebae in the presence of RNA and protein synthesis inhibitors. J. Cell Biol. 49, 221–226 (1971)
Flickinger, C. J.: The influence of inhibitors of energy metabolism on the formation of Golgi bodies in amebae. Exp. Cell Res. 73, 154–160 (1972)
Franke, W. W., Eckert, W. A.: Cytomembrane differentiation in a ciliate, Tetrahymena pyriformis. II. Bifacial cisternae and tubular formations. Z. Zellforsch. 122, 244–253 (1971)
Franke, W. W., Eckert, W. A., Krien, S.: Cytomembrane differentiation in a ciliate, Tetrahymena pyriformis. I. Endoplasmic reticulum and dictyosomal equivalents. Z. Zellforsch. 119, 577–604 (1971)
Frankel, J.: The relation of protein synthesis to cell division and oral development in synchronized Tetrahymena pyriformis GL-C: An analysis employing cycloheximide. J. Cell Physiol. 74, 135–148 (1969)
Gordon, G. B., Miller, L. R., Bensch, K. G.: Fixation of tissue culture cells for ultrastructure cytochemistry. Exp. Cell Res. 31, 440–443 (1963)
Karnovsky, M. J.: The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J. Cell Biol. 35, 213–236 (1967)
Levy, M. R., Elliott, A. M.: Biochemical and ultrastructural changes in Tetrahymena pyriformis during starvation. J. Protozool. 15, 208–222 (1968)
McKanna, J. A.: Cyclic membrane flow in the ingestive-digestive system of peritrich protozoans. I. Vesicular fusion at the cytopharynx. II. Cup-shaped coated vesicles. J. Cell Sci. 13, 663–686 (1973)
Morré, D. J., Mollenhauer, H. H., Bracker, C. E.: Origin and continuity of Golgi apparatus. In: Results and problems in cell differentiation. Origin and continuity of cell organelles, (ed. J. Reinert and H. Ursprung), vol. II, p. 82–126. New York: Springer-Verlag 1971
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)
Rosenbaum, J. L., Carlson, K.: Cilia regeneration in Tetrahymena and its inhibition by colchicine. J. Cell Biol. 40, 415–425 (1969)
Tyler, L., Wolfe, J.: Control of cell fusion in conjugating Tetrahymena. J. Protozool. 19, 42A-43A (1972)
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Supported by grants from NSF (GB-32285) and the American Cancer Society (E-500).
The authors acknowledge the technical assistance of Mrs. Sue Thompson.
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Flickinger, C.J., Murray, R.L. Cytoplasmic membrane changes in conjugating Tetrahymena . Cell Tissue Res. 153, 357–364 (1974). https://doi.org/10.1007/BF00229164
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DOI: https://doi.org/10.1007/BF00229164