Abstract
In vitro pollen germination of five species and two interspecific hybrids from the genus Brassica was tested in four media. Genetically fixed differences in the demands for optimal pollen germination among species were found. The experiments were designed to define optimal content of mineral salts, sugar, and PEG for every investigated species or hybrids. The differences found among species are discussed in relation to the evolutionary trend.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Blow AMJ, Botham GM, Fisher D, Goodall AH, Tilcock CPS, Lucy JA (1978) Water and calcium ions in cell fusion induced by polyethylene glycol. FEBS Lett 94:305–310
Carter AL, Neilly T (1975) Effect of increased humidity on pollen growth and seed set following self pollination in Brussels sprout (Brassica oleracea var. gemmifera) Euphytica 24:805–813
Digonnet-Kerhoas C, Gay G, Duplan JC, Dumas C (1989) Viability of Cucurbita pepo pollen: biophysical and structural data. Planta 179:165–170
Dumas Ch, Bowman RB, Gaude T, Guilly CM, Heizmann P, Roeckel P, Rougier M (1988) Stigma and stigmatic secretion reexamined. Phyton 282:193–200
Ferrari TE, Wallance DH (1975) Germination of Brassica pollen and expression of incompatibility in vitro. Euphytica 24:757–765
Gaude T, Dumas C (1984) A membrane like structure on the pollen wall surface in Brassica. Ann Bot 54:821–825
Hahn-Hagerdal B, Hosono K, Zachrisson A, Bornman CH (1986) Polyethylene glycol and electric field treatment of plant protoplasts: characterization of some membrane properties. Physiol Plant 67:359–364
Hodgkin T (1987) A procedure suitable for in vitro pollen selection in Brassica oleracea. Euphytica 36:153–159
Hodgkin T (1990) In vitro pollen selection in Brassica napus L. for resistance to phytotoxic compounds from Alternaria brassicola (Schw.) Wilts. Sex Plant Reprod 3:116–120
Hodgkin T, Lyon GD (1983) Detection of pollen germination inhibitors in Brassica oleracea tissue extracts. Ann Bot 52:781–789
Hoekstra FA, Bruinsma J (1979) Protein synthesis of binucleate and trinucleate pollen and its relationships to tube emergence and growth. Planta 146:559–566
Hosaka K, Kianian SF, McGrath JM, Quiros CF (1990) Development and chromosome localization of genome specific DNA markers of Brassica and evolution of amphidiploids and n = 9 diploid species. Genome 33:131–142
Jandurová O, Pavlík M (1991) Differences in requirement of medium composition for pollen germination in vitro at some cultivars of Brassica napus. Sb Vys Sk Zemed Praha 53:257–267
Lakshmikumaran M, Ranade SA (1990) Isolation and characteristic of highly repetitive DNA of Brassica campestris. Plant Mol Biol 14:447–448
Mascarenhas JP (1989) The male gametophyte of flowering plants. Plant Cell 1:657–664
McConchie CA, Russel SD, Dumas C, Tuohy M, Knox RB (1987) Quantitative cytology of the sperms of Brassica campestris and B. oleracea. Planta 170:446–452
Mulcahy DL, Ottaviano EM (1983) Pollen: biology and implications for plant breeding. Elsevier, New York
Nasrallah JB, Yu SM, Nasrallah ME (1988) Self incompatibility genes of Brassica oleracea. Expression, isolation and structure. Proc Natl Acad Sci USA 85:5551–5555
Quiros CF, Ochoa O, Kianian SF, Douches DS (1987) Analysis of Brassica oleracea genome by the generation of B. campestris-oleracea chromosome addition lines: characterization by isozymes and rDNA genes. Theor Appl Genet 74:758–766
Quiros CF, Ochoa O, Douches DS (1988) Exploring the role of x = 7 species in Brassica evolution: hybridization with B. nigra and B. oleracea. J Hered 79:351–358
Robbelen G (1960) Beiträge zur Analyse des Brassica genomes. Chromosoma 11:205–228
Roberts IN, Stead AD, Ockendon DJ, Dickinson HG (1980) Pollen stigma interactions in Brassica oleracea. Theor Appl Genet 58:241–246
Roberts IN, Gaude TC, Harrod G, Dickinson HG (1983) Pollenstigma interactions in Brassica oleracea; a new pollen germination medium and its use in elucidating the mechanism of self incompatibility. Theor Appl Genet 65:231–238
Sarker RH, Ellenman CJ, Dickinson HG (1988) Control of pollen hydration in Brassica requires continued protein synthesis and glycosylation is necessary for intraspecific incompatibility. Proc Natl Acad Sci USA 85:4340–4344
Singh A, Perdue TD, Paollilo DJ Jr (1989) Pollen-pistil interactions in Brassica oleracea: cell calcium in self and cross pollen grains. Protoplasma 151:57–61
Swanson EB, Conmans MP, Brown GL, Patel JD, Beversdorf WD (1988) The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts. Plant Cell Rep 83–87
Tilcock CPS, Fischer D (1979) Interaction of phospholipids membrane with polyethylene glycols. Biochim Biophys Acta 775:446–448
UN (1935) Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn J Bot 7:389–452
Willing RP, Mascarenhas JP (1984) Analysis of the complexity and diversity of mRNAs from pollen and shoots of Tradescantia. Plant Physiol 75:865–868
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jandurová, O.M., Pavlík, M. In vitro pollen germination of species and two interspecific hybrids from the genus Brassica . Sexual Plant Reprod 8, 33–36 (1995). https://doi.org/10.1007/BF00228760
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00228760