Abstract
The hydroxyproline content of developing Zea mays (maize) pollen was determined. The level of hydroxyproline in uninucleate microspores early in pollen development was low (0.004% of dry weight). In contrast, mature pollen is enriched for this amino acid (0.1% of dry weight). In mature pollen, 90% of the hydroxyproline is in the soluble fraction. Upon in vitro pollen germination, hydroxyproline associated with the insoluble fraction increased from 10% to 26% of the total hydroxyproline. Antibodies specific to extensins and arabinogalactan proteins (AGPs), two major classes of hydroxyproline-containing proteins, recognized two distinct groups of proteins in maize pollen by Western analysis. The two types of pollen hydroxyproline-containing proteins could also be distinguished based on their behavior upon anion exchange chromatography.
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Rubinstein, A.L., Prata, R.T.N. & Bedinger, P.A. Developmental accumulation of hydroxyproline and hydroxyproline-containing proteins in Zea mays pollen. Sexual Plant Reprod 8, 27–32 (1995). https://doi.org/10.1007/BF00228759
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DOI: https://doi.org/10.1007/BF00228759