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Reduced cardiac myofibrillar Mg-ATPase activity without changes in myosin isozymes in patients with end-stage heart failure

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Abstract

In this study we tested the hypothesis that reduced myofibrillar ATPase activities in end-stage heart failure are associated with a redistribution of myosin isozymes. Cardiac myofibrils were isolated from left ventricular free wall from normal human hearts and hearts at end-stage heart failure caused by coronary artery diseases, cardiomyopathy or immunological rejection. The hearts had been excised in preparation for a heart transplant. Myofibrillar Ca2−-dependent Mg-ATPase and myosin Ca−- and K−EDTA-ATPase activities were compared. Possible changes in myosin isozyme distribution in the diseased heart were investigated using polyacrylamide gel electrophoresis of native myosin in the presence of pyrophosphate. Significant reduction in myofibrillar Ca2+-dependent Mg-ATPase with no changes in the sensitivity of the myofibrils to Ca+ was observed in heart with coronary artery diseases (25.2 to 27.1% at pCa 5.83 to pCa 5.05), cardiomyopathy (21.1 to 25.5% at pCa 5.41 to pCa 5.05), and in the immunologically rejected heart (18.4 to 22.8% at pCa 5.41 to pCa 5.05). Significantly lower myosin Ca2+-ATPase was observed with coronary artery diseases only and myosin K-EDTA activities did not differ in diseased and normal hearts. Polyacrylamide gel electrophoresis of native myosin from the normal and three models of end-stage heart failure revealed two distinct bands in the human left ventricle and one diffuse band in the human right atria. No apparent differences in myosin isoenzyme pattern were observed between the normal and diseased hearts. Further evaluation is needed to clarify the ATPase nature of the two bands.

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Authors and Affiliations

  1. Sterling Research Group, Rensselaer, NY

    Adawia A. Alousi, Alison M. Grant & John R. Etzler

  2. Cardiac Transplantation Program, University of Cincinnati, Cincinnati, Ohio, USA

    Barry R. Cofer, Joanna Van der Bel-Kahn & David Melvin

Authors
  1. Adawia A. Alousi
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  2. Alison M. Grant
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  3. John R. Etzler
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  4. Barry R. Cofer
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  5. Joanna Van der Bel-Kahn
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  6. David Melvin
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Alousi, A.A., Grant, A.M., Etzler, J.R. et al. Reduced cardiac myofibrillar Mg-ATPase activity without changes in myosin isozymes in patients with end-stage heart failure. Mol Cell Biochem 96, 79–88 (1990). https://doi.org/10.1007/BF00228455

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  • DOI: https://doi.org/10.1007/BF00228455

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