Molecular and Cellular Biochemistry

, Volume 105, Issue 2, pp 119–125 | Cite as

Brown adipose tissue mitochondria are cytochrome c — subsaturated

  • Amos S. Gaikwad
  • T. Ramasarma
  • C. K. Ramakrishna Kurup


The oxidative activity of mitochondria freshly isolated from brown adipose tissue of rats was stimulated two-fold on the addition of small concentrations of exogenous cytochrome c to the reaction medium. Loss of membrane-bound cytochrome c did not occur during isolation of mitochondria. Estimation of the high-affinity binding sites on the organelle membrane indicated that less than a third of these sites remained saturated with cytochrome c. The pigment is thus shown to be a functionally limiting electron transport component in brown adipose tissue.

Key words

BAT mitochondria electron transport stimulation cytochrome c binding sites 


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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Amos S. Gaikwad
    • 1
  • T. Ramasarma
    • 1
  • C. K. Ramakrishna Kurup
    • 1
  1. 1.Department of BiochemistryIndian Institute of ScienceBangaloreIndia

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