Abstract
The isolation of viable egg cells of wheat has been achieved without enzymatic maceration of the ovules. 2,4-D applied to the stigmas resulted 3 to 7 days later in soft ovule tissues which disintegrated upon mechanical manipulation. The isolated egg cells were viable even 2 h after isolation. Their morphology corresponded to that of the in situ egg cells. The mean isolation frequency was 20% (two egg cells per ten ovules).
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Kovács, M., Barnabás, B. & Kranz, E. The isolation of viable egg cells of wheat (Triticum aestivum L.). Sexual Plant Reprod 7, 311–312 (1994). https://doi.org/10.1007/BF00227715
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DOI: https://doi.org/10.1007/BF00227715