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Differences in salt solubility, DNase II sensitivity and template activity of chromatin from antibody synthesizing spleen cells and myeloma cells RPC 5 and ABPC 22

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Summary

Chromatin from myeloma cells RPC 5 and ABPC 22, and from spleen and liver cells of immunized rats and mice, and mice bearing tumours, was fractionated into three parts: 0.35 M NaCl-soluble, 2 M NaCl-soluble and residual. The residual fraction from myeloma cells differed from that of immunized spleen cells, described previously as containing unique sequences (5), in that it has higher protein and DNA levels, lower DNase II sensitivity and lower template activity.

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Authors and Affiliations

  1. Polish Academy of Sciences, Institute of Human Genetics, Swiecickiego 6, 60-781, Poznań, Poland

    E. M. Rakowicz-Szulczyńska & A. Horst

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  1. E. M. Rakowicz-Szulczyńska
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  2. A. Horst
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Rakowicz-Szulczyńska, E.M., Horst, A. Differences in salt solubility, DNase II sensitivity and template activity of chromatin from antibody synthesizing spleen cells and myeloma cells RPC 5 and ABPC 22. Mol Cell Biochem 48, 71–76 (1982). https://doi.org/10.1007/BF00227607

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  • DOI: https://doi.org/10.1007/BF00227607

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