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Removal of beta-lactam antibiotics during growth of Escherichia coli strains hosting pUR and pEX vectors

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Biotechnology Techniques

Summary

Beta-lactam antibiotics supplied at 200 μg/mL were rapidly removed from culture fluids of E. coli strains hosting pUR and pEX vectors, indicating that a substantial period of growth occurred in the absence of positive selection. The retention period of the drugs depended on the specific activity and type of the beta-lactamase, the nature of the antibiotic and growth rate of the host.

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Authors and Affiliations

  1. Division of Chemical and Wood Technology, CSIRO, Private Bag 10, 3168, Clayton, Australia

    G. W. Kemp & M. L. Britz

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  1. G. W. Kemp
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  2. M. L. Britz
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Kemp, G.W., Britz, M.L. Removal of beta-lactam antibiotics during growth of Escherichia coli strains hosting pUR and pEX vectors. Biotechnol Tech 1, 157–162 (1987). https://doi.org/10.1007/BF00227553

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  • DOI: https://doi.org/10.1007/BF00227553

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