Summary
A method for the on-line estimation of biomass is proposed. The method is based on a liquid-phase dynamic mass balance for oxygen during a sequence of “no aeration” transients. The method has the advantage that no samples need be withdrawn and the biomass need not be homogeneously distributed in the vessel. The technique thus has applications in the estimation of immobilised biomass and plant organ culture, e.g. “hairy roots”.
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Wilson, P.D.G. On-line estimation of biomass using dynamic oxygen balancing. Biotechnol Tech 1, 151–156 (1987). https://doi.org/10.1007/BF00227552
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DOI: https://doi.org/10.1007/BF00227552