Summary
Protein extracts from four self-incompatible genotypes of Phalaris coerulescens were subjected to analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and ultra-thin isoelectric focusing. A comparison between stigma, root and leaf extracts showed that there was no significant difference in electrophoretic or isoelectric focusing patterns between the genotypes for both root and leaf proteins. However, stigma protein patterns did vary between genotypes especially within the molecular weight region of 43 000–97 000 and within the pI range 5–7. The stigma-specific changes strongly suggest a link between the self-incompatible genotype and these stigma proteins. However, because there are two loci involved, it is not yet possible to precisely assign particular proteins to each S- or Z-allele.
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Tan, L.W., Jackson, J.F. Stigma proteins of the two loci self-incompatible grass Phalaris coerulescens . Sexual Plant Reprod 1, 25–27 (1988). https://doi.org/10.1007/BF00227018
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DOI: https://doi.org/10.1007/BF00227018