Abstract
Four methods for classifying cultivated rices (Oryza sativa L.) (including IR varieties) into indica and japonica types — waxy gene product in endosperm starch, glutelin α3 molecular weight in milled rice, RFLP polymorphism at the Wx locus and Glaszmann's isozyme method — were compared. On the basis of the two endosperm traits and the RFLP method Glaszmann's group 1 (indica) was classified as mainly indica and intermediate groups 2, 3 and 4 as exclusively indica. However, the endosperm traits classified Glaszmann's group 5 as mainly indica, while the RFLP method classified it as japonica. The RFLP waxy gene probe was closest to the isozyme method in classifying group 6 as japonicas; the waxy gene product gave mainly indica reaction even in group 6, and the glutelin α3 method was intermediate. All IR rices were classified as being indica on the basis of Wx gene product and by Glaszmann's method, but a few were classified as japonica by the glutelin α3 method and by the RFLP waxy gene probe.
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Glaszmann JC (1987) Isozymes and classification of Asian rice varieties. Theor Appl Genet 74:21–30
International Rice Research Institute (1991) Program report for 1990. IRRI, Manila, Philippines, pp 210–212
Juliano BO, Perez CM, Blakeney AB, Castillo DT, Kongseree N, Laignelet B, Lapis ET, Murty VVS, Paule CM, Webb BD (1981) International cooperative testing on the amylose content of milled rice. Starch/Staerke 33:157–162
Kagawa H, Hirano H, Kikuchi F (1988) Variation of glutelin seed storage protein in rice (Oryza sativa L.). Jpn J Breed 38:327–332
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Ogawa M, Kumamaru T, Satoh H, Iwata N, Omura T, Kasai Z, Tanaka K (1987) Purification of protein body-I of rice seed and its polypeptide composition. Plant Cell Physiol 28:1517–1527
Sano Y, Katsumata M, Okuno K (1986) Genetic studies on speciation in cultivated rice. 5. Inter- and intraspecific differentiation in the waxy gene expression of rice. Euphytica 35:1–9
Villareal CP, Juliano BO (1989) Comparative levels of waxy gene product of endosperm starch granules of different rice ecotypes. Starch/Staerke 41:369–371
Wang ZY, Tanksley SD (1989) Restriction fragment length polymorphism in Oryza sativa L. Genome 32:1113–1118
Williams MNV, Pande N, Nair S, Mohan M, Bennett J (1991) Restriction fragment length polymorphism analysis of polymerase chain reaction products amplified from mapped loci of rice (Oryza sativa L.) genomic DNA. Theor Appl Genet 82:489–498
Zhang Q, Saghai Maroof MA, Lu TY, Shen BZ (1992) Genetic diversity and differentiation of japonica and indica rices detected by RFLP analysis. Theor Appl Genet 83:495–499
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Communicated by G. S. Khush
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Resurreccion, A.P., Villareal, C.P., Parco, A. et al. Classification of cultivated rices into indica and japonica types by the isozyme, RFLP and two milled-rice methods. Theoret. Appl. Genetics 89, 14–18 (1994). https://doi.org/10.1007/BF00226976
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DOI: https://doi.org/10.1007/BF00226976