Skip to main content

Engineering 2,4-D resistance into cotton

Theoretical and Applied Genetics volume 83, pages 645–649 (1992)Cite this article

Summary

To reduce damage by drift-levels of the herbicide 2,4-dichlorophenoxyacetic acid, we have engineered the 2,4-D resistance trait into cotton (Gossypium hirsutum L.). The 2,4-D monooxygenase gene tfdA from Alcaligenes eutrophus plasmid pJP5 was isolated, modified and expressed in transgenic tobacco and cotton plants. Analyses of the transgenic progeny showed stable transmission of the chimeric tfdA gene and production of active 2,4-D monooxygenase. Cotton plants obtained were tolerant to 3 times the field level of 2,4-D used for wheat, corn, sorghum and pasture crops.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  • Amy PS, Schulke JW, Frazier LM, Seidler RJ (1985) Characterization of aquatic bacteria and cloning of genes specifying partial degradation of 2,4-Dichlorophenoxyacetic acid. Appl Environ Microbiol 49:1237–1245

    Google Scholar 

  • Assaad FF, Signer ER (1990) Cauliflower mosaic virus P35S promoter activity in Escherichia coli. Mol Gen Genet 223:517–520

    Google Scholar 

  • Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 12:8711–8721

    Google Scholar 

  • Don RH, Pemberton JM (1981) Properties of six pesticide degradation plasmids isolated from Alcaligenes paradoxus and Alcaligenes eutrophus. J Bacteriol 145:681–686

    Google Scholar 

  • Horsch RB, Fry JE, Hoffman NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231

    Google Scholar 

  • Lyon BR, Llewellyn DJ, Huppatz JL, Dennis ES, Peacock WJ (1989) Expression of a bacterial gene in transgenic tobacco plants confers resistance to the herbicide 2,4-dichlorophenoxyacetic acid. Plant Mol Biol 13:533–540

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Ow DW, Wood KV, DeLuca M, deWet JR, Helinski DR, Howell SH (1986) Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science 234:856–859

    Google Scholar 

  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer directed amplication of DNA with a thermostable DNA polymerase. Science 239:487–491

    Google Scholar 

  • Stewart JMcD, Hsu CL (1977) In ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.). Planta 137:113–117

    Google Scholar 

  • Streber WR, Willmitzer L (1989) Transgenic tobacco plants expressing a bacterial detoxifying enzyme are resistant to 2,4-D. Biotechnology 7:811–816

    Google Scholar 

  • Streber WR, Timmis KN, Zenk MH (1987) Analysis, cloning, and high-level expression of 2,4-dichlorophenoxyacetate monooxygenase gene tfdA of Alcaligenes eutrophus JMP134. J Bacteriol 169:2950–2955

    Google Scholar 

  • Takebe I, Labib G, Melchers G (1971) Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Naturwissenschaften 88:318–320

    Google Scholar 

  • Trolinder N, Goodin JR (1987) Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum). Plant Cell Reports 6:231–234

    Google Scholar 

  • Umbeck P, Johnson G, Barton K, Swain W (1987) Genetically transformed cotton (Gossypium hirsutum L.) plants. Bio/Technology 5:263–266

    Google Scholar 

Download references

Author information

Affiliations

  1. Plant Gene Expression Center, USDA/ARS, and Department of Plant Pathology, University of California at Berkeley, 800 Buchanan St., 94710, Albany, CA, USA

    C. Bayley, C. Ray, M. Morgan & D. W. Ow

  2. Cropping Systems Research Laboratory, USDA/ARS, Route 3, Box 213, 79401, Lubbock, TX, USA

    N. Trolinder & J. E. Quisenberry

Authors
  1. C. Bayley
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Trolinder
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Ray
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Morgan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. E. Quisenberry
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. W. Ow
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by P. Maliga

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bayley, C., Trolinder, N., Ray, C. et al. Engineering 2,4-D resistance into cotton. Theoret. Appl. Genetics 83, 645–649 (1992). https://doi.org/10.1007/BF00226910

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00226910

Key words

  • Genetic modification
  • Gene transfer
  • tfdA
  • Gossypium hirsutum
  • Herbicide