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Analysis of DNA from a Beta procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance

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Summary

We have begun to apply techniques for the preparation and anaylsis of large DNA segments from sugar beet (Beta vulgaris) addition lines carrying a mitotically stable chromosome fragment from B. procumbens that confers monogenic resistance to the nematode Heterodera schachtii, with a view towards isolating the resistance gene. DNA probes specific for this chromosome fragment were selected, and various methods for cloning genome-specific fragments, including probes from megabase DNA separated in pulsed-field slab gels, are compared. Probes that display high homology to B. procumbens have been used for hybridization of a representative genomic library and for initial step in mapping the chromosome fragment via pulsed-field gel electrophoresis after restriction with infrequently cutting enzymes. Our data indicate that DNA molecules from the entire chomosome fragment can be separated from protoplast DNA lysates.

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Authors and Affiliations

  1. Botanical Institute of the Ludwig Maximilians University Munich, Menzinger Straße 67, 8000, Munich 19, FRG

    C. Jung, M. Kleine, E. Fischer & R. G. Herrmann

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  1. C. Jung
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  2. M. Kleine
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  3. E. Fischer
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  4. R. G. Herrmann
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Communicated by G. Wenzel

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Jung, C., Kleine, M., Fischer, E. et al. Analysis of DNA from a Beta procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance. Theoret. Appl. Genetics 79, 663–672 (1990). https://doi.org/10.1007/BF00226881

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  • DOI: https://doi.org/10.1007/BF00226881

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