Skip to main content
Log in

Biochemical characterization of lines descended from 8x triticale x 4x triticale cross

  • Originals
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

Fifty-two progenies originating from a cross between 8x and 4x triticale were submitted to cytogenetic analyses and to various electrophoretic studies [high-molecular-weight (HMW) glutelins, HMW secalins, γ-secalins, α and ω-gliadins, β-amylases] to identify new genetic structures, more specifically the input of the D genome in a genetic context other than the wheat one. Markers of the rye genome (HMW and ω-secalins) were identified in all of the triticale lines, but they originated either from the 4x or from the 8x parent, or from both. Chromosomes 4A, 1B, and 2R, present in both parents, showed the same banding patterns in all progenies. Chromosomes 1R and 5R, present in both parents, showed heterogeneous labelling. The expression of chromosomes 6A, 1D, and 4D, present in the 8x parent only, was more complex with a possible involvement of a regulatory system. Several hexaploid progenies had introgressed part of the D genome, suggesting that crossing 8x and 4x triticale was a practicable approach for transferring D chromosomes into hexaploid triticale.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Artemova NV (1982) Chromosomal control of alcohol deshy-drogenase, esterase and γ-amylase iso-enzymes in different rye cultivars. Genetika 18:661–667

    Google Scholar 

  • Autran JC, Berrier R (1984) Durum wheat functional subunits revealed through heat treatment. Biochemical and genetic implications. In: Graveland A, Moonen JHE (eds) Proc 2nd Int Workshop Gluten Proteins, T.N.O., Wageningen, The Netherlands, pp 175–183

    Google Scholar 

  • Bernard M, Bernard S (1978) Methods of gene transfer from bread wheat and rye to hexaploid triticale. In: Sanchez-Monge E, Garcia Olmedo F (eds) Interspecific hybridization in plant breeding, Proc 8th Eucarpia Congr. Madrid, 1977

    Google Scholar 

  • Bernard M, Bernard S (1985) Meiotic pairing in hybrids between tetraploid triticales and related species: new elements concerning the chromosome constitution of tetraploid triticale. Theor Appl Genet 70:390–399

    Google Scholar 

  • Bernard M, Bernard S (1987) Creating new forms of 4x, 6x and 8x primary triticale associating both complete R and D genomes. Theor Appl Genet 74:55–59

    Google Scholar 

  • Bernard M, Saigne B (1977) Etude cytogénétique de F1 et F2 (Triticale hexaploïde x Seigle): mise en évidence d'une inhibition du système diploïdisant du blé tendre. Ann Amél Plant 27:737–748

    Google Scholar 

  • Bernard M, Autran JC, Joudrier P (1977) Possibilités d'identification de certains chromosomes de seigle à l'aide de marqueurs biochimiques. Ann Amél Plant 27:355–362

    Google Scholar 

  • Bernard M, Gay G, Saigne B (1985a) Study of the fertility and chromosome behaviour of 3 successive generations obtained following crosses between octoploid and tetraploid triticale. In: Bernard M, Bernard S (eds) Genetic and breeding of triticale. Proc 3rd Eucarpia Meeting on Triticale, Clermont-Ferrand, France, pp 245–257

  • Bernard M, Bernard S, Saigne B (1985) Tetraploid triticales: investigations on their genome and chromosome constitution. In: Bernard M, Bernard S (eds) Genetic and breeding of triticale. Proc 3rd Eucarpia Meeting on Triticale, Clermont-Ferrand, France, pp 277–287

  • Branlard G, Le Blanc A (1984) Les sous-unités gluténines de haut poids moléculaire des blés tendres et des blés durs cultivés en France. Agronomie 5:467–477

    Google Scholar 

  • Bushuk W, Zillman RR (1978) Wheat cultivar identification by gliadin electrophoregrams. I. Apparatus, method and nomenclature. Can J Plant Sci 58:505–515

    Google Scholar 

  • Chen CH, Bushuk W (1970) Nature of proteins in triticale and its parental species. III. A comparison of their electrophoretic patterns. Can J Plant Sci 50:25–30

    Google Scholar 

  • Field JM, Shewry PR (1987) Prolamin subunit interaction in hexaploid and octoploid triticales and their wheat and rye parents. J Cereal Sci 6:199–210

    Google Scholar 

  • Field IM, Shewry PR, Miflin BJ, March IF (1982) The purification and characterization of homologous high molecular weight storage proteins from grain of wheat, rye and barley. Theor Appl Genet 62:329–336

    Google Scholar 

  • Gustafson JP, Lukaszewski AJ, Robertson K (1985) Chromosome substitution and modifications in hexaploid triticale: a re-evaluation. In: Bernard M, Bernard S (eds) Genetics and breeding of triticale. 3rd Eucarpia Meeting on Triticale, Clermont-Ferrand, France, pp 15–17

  • Joudrier P (1974) Specificité génétique de la γ-amylase chez Triticum aestivum: existence de cinq types variétaux de zymogrammes. CR Acad Sci Paris 278D:1777–1880

    Google Scholar 

  • Joudrier P, Cauderon Y (1976) Localisation chromosomique de gènes contrôlant la synthèse de certains constituants γ-amylasiques du grain de blé tendre. CR Acad Sci Paris 282D:115–118

    Google Scholar 

  • Kasarda DD, Bernardin JE, Qualset CO (1976) Relationship of gliadin protein components to chromosomes in hexaploid wheats (Triticum aestivum L.). Proc Natl Acad Sci USA 73:3646–3650

    Google Scholar 

  • Kasarda DD, Autran JC, Lew EJL, Nimmo CC, Shewry PR (1983) N-terminal amino acid sequences of ω-gliadins and ω-secalins. Implications for the evolution of prolamin genes. Biochim Biophys Acta 747:138–150

    Google Scholar 

  • Kreis M, Shewry PR, Forde BG, Forde J, Miflin BJ (1985) Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat, barley and rye. Oxford Surv Plant Mol Cell Biol 2:253–317

    Google Scholar 

  • Lawrence GJ, Shepherd KW (1981) Chromosomal location of genes controlling seed proteins in species related to wheat. Theor Appl Genet 59:25–31

    Google Scholar 

  • Lei MG, Reeck GR (1986) Two-dimensional electrophoretic analysis of kernel proteins of triticales and of their parental durum wheats and ryes. Cereal Chem 63:116–123

    Google Scholar 

  • Lukaszewski AJ, Apolinarska B, Gustafson JP (1987) Introduction of the D-genome chromosomes from bread wheat into hexaploid triticale with a complete rye genome. Genome 29:425–430

    Google Scholar 

  • Lupano CE, Anon MC (1985) Characterization of triticale protein. Cereal Chem 62:174–178

    Google Scholar 

  • Orth RA, Dronzek BL, Bushuk W (1974) Studies of glutenin. VII. Inheritance of its physicochemical factor in triticale. Cereal Chem 51:281–288

    Google Scholar 

  • Payne PI, Corfield KG (1979) Subunit composition of wheat glutenin proteins, isolated by gel filtration in a dissociating medium. Planta 145:83–88

    Google Scholar 

  • Payne PI, Lawrence GJ (1983) Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11:29–33

    Google Scholar 

  • Preston KR, Woodbury W, Orth RA, Bushuk W (1975) Comparison of gliadin and glutenin subunits in the triticinae by SDS-polyacrylamide gel electrophoresis. Can J Plant Sci 55:667–672

    Google Scholar 

  • Shewry PR, Miflin BJ (1984) Seed storage proteins of economically important cereals. In: Pomeranz Y (ed) Advances in cereal science and technology, vol 7. AACC, St Paul MN, pp 1–83

    Google Scholar 

  • Shewry PR, Field JM, Lew EJL, Kasarda DD (1982) The purification and characterization of two groups of storage proteins (secalins) from rye (Secale cereale L.). J Exp Bot 33:261–268

    Google Scholar 

  • Shewry PR, Parmar S, Miflin BJ (1983a) Extraction, separation and polymorphism of the prolamin storage proteins (secalins) of rye. Cereal Chem 60:1–6

    Google Scholar 

  • Shewry PR, Kreis M, Burgess SR, Parmar S, Miflin BJ (1983b) The synthesis and deposition of the prolamin storage proteins (secalins) of rye. Planta 159:439–445

    Google Scholar 

  • Shewry PR, Bradberry D, Franklin J, White RD (1984) The chromosomal locations and linkage relationships of the structural genes for the prolamin storage proteins (secalins) of rye. Theor Appl Genet 69:63–69

    Google Scholar 

  • Singh NK, Shepherd KW (1984) Mapping of the genes controlling high-molecular-weight glutelin subunits of rye on the long arm of chromosome 1R. Genet Res Camb 4:117–123

    Google Scholar 

  • Skovmand B, Braun HJ, Fox PN (1985) Comparison of agronomic and quality characteristics of complete and substituted hexaploid spring triticales. In: Bernard M, Bernard S (eds) Genetic and breeding of triticale. Proc 3rd Eucarpia Meeting on Triticale, Clermont-Ferrand, France, pp 29–34

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by G. Wenzel

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bernard, M., Bernard, S., Joudrier, P. et al. Biochemical characterization of lines descended from 8x triticale x 4x triticale cross. Theoret. Appl. Genetics 79, 646–653 (1990). https://doi.org/10.1007/BF00226878

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00226878

Key words

Navigation