Summary
The change of phenotype from sterility to fertility for some cmsT callus tissue culture regenerated plants and their progenies has been correlated with changes in their mitochondrial genome. Those changes that have been analyzed here are the result of recombination events. Two different sets of repeated sequences have been found to be involved in those recombination events. The most common one is a recombination through a 127-bp repeat between various independently isolated revertants. The second one is a recombination through a 58-bp repeat. In every case the products of recombination containing the urf13 gene have been deleted.
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References
Abbott AG, Fauron CM-R (1986) Structural alterations in a transcribed region of the T-type cytoplasmic male-sterile maize mitochondrial genome. Curr Genet 10:777–783
Brettell RIS, Goddard BVD, Ingram DS (1979) Selection of TMS-cytoplasm maize tissue cultures resistant to Drechslera maydis T-toxin. Maydica 24:203–213
Brettell RIS, Thomas E, Ingram DS (1980) Reversion of Texas male-sterile cytoplasm maize in culture to give fertile, T-toxin resistant plants. Theor Appl Genet 58:55–58
Brettell RIS, Conde MF, Pring DR (1982) Analysis of mitochondrial DNA from four fertile maize lines obtained from a tissue culture carrying Texas cytoplasm. Maize Genet Coop New Letter 56:1314
Chen EJ, Seeburg PH (1985) Supercoil sequencing: a fast simple method for sequencing plasmid DNA. DNA 4:165–170
Dale RMK, Mendu N, Ginsburg H, Kridl JC (1984) Sequence analysis of the maize mitochondrial 26S rRNA gene and flanking regions. Plasmid 11:141–150
Dewey RE, Levings CS III, Timothy DH (1986) Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm (cmsT). Cell 44:439–449
Dewey RE, Timothy DH, Levings CS III (1987) A mitochondrial proteins associated with cytoplasmic male sterility in the T-cytoplasm of maize. Proc Natl Acad Sci USA 84:5374–5378
Dewey RE, Siedon JN, Timothy DH, Levings CS III (1988) A 13-kilodalton maize mitochondrial protein in E. coli confers sensitivity to Bipolaris maydis toxin. Science 239:293–295
Dixon LK, Leaver CJ, Brettell RIS, Gengenbach BG (1982) Mitochondrial sensitivity to Drechslera maydis T-toxin and the synthesis of a variant mitochondrial polypeptide in plants derived from maize tissue cultures with Texas malesterile cytoplasm. Theor Appl Genet 63:75–80
Fauron CM-R, Abbott AG, Brettell R, Gesteland RF (1987) Maize mitochondrial DNA rearrangements between the normal type, the Texas male-sterile cytoplasm and a fertile revertant cmsT regenerated plant. Curr Genet 11:339–346
Fauron CM-R, Havlik M, Lonsdale D, Nichols L (1989) Mitochondrial genome organization of the maize cytoplasmic male-sterile type T. Mol Gen Genet 216:395–401
Fauron CM-R, Havlik M, Brettell RIS (1990) The mitochondrial genome organization of a maize fertile cmsT revertant line is generated through recombination between two sets of repeats. Genetics 124:423–428
Forde BG, Leaver CJ (1980) Nuclear and cytoplasmic genes controlling synthesis of variant mitochondrial polypeptides in male-sterile maize. Proc Natl Acad Sci USA 77:418–422
Forde BG, Oliver RJC, Leaver CJ (1978) Variation in mitochondrial translation products associated with male-sterile cytoplasms in maize. Proc Natl Acad Sci USA 75:3841–3845
Gengenbach BG, Green CE, Donovan CM (1977) Inheritance of selected pathotoxin resistant in maize plants regenerated from cell cultures. Proc Natl Acad Sci USA 74:5113–5117
Gengenbach BG, Connelly JA, Pring DR, Conde MF (1981) Mitochondrial DNA variation in maize plants regenerated during tissue culture selection. Theor Appl Genet 59:161–167
Hohn B, Collins J (1980) A small cosmid for efficient cloning of large DNA fragment. Gene 11:291–298
Kemble RJ, Gunn RE, Flavell RB (1980) Classification of normal and male-sterile cytoplasms in maize. Electrophoretic analysis of DNA species in mitochondria. Genetics 95:451–458
Kemble RJ, Flavell RB, Brettell RIS (1982) Mitochondrial DNA analyses of fertile and sterile maize plants derived from tissue culture with the Texas male sterile cytoplasm. Theor Appl Gen 62:213–217
Pring DR, Gengenbach BG, Wise RP (1988) Recombination is associated with polymorphism of the mitochondrial genomes of maize and sorghum. Philos Trans R Soc London Ser B 319:187–198
Qin J, Fauron CM-R, Brettell RIS, Milhous M, Abbott A (1987) Toxin resistance and/or male fertility reversion is correlated with defined transcription changes in the 1.5-kb AvaI region of cmsT. Nucleic Acids Res 15:6091
Rottman WH, Brears T, Hodge TP, Lonsdale DM (1987) Mitochondrial gene is lost via homologous recombination during reversion of cmsT maize to fertility. EMBO J 6:1541–1546
Umbeck PF, Gengenbach BG (1983) Reversion of male-sterile T-cytoplasm maize to male fertility in tissue culture. Crop Sci 23:584–588
Wahl GM, Lewis KA, Ruiz JC, Rothenberg B, Zhao J, Evans GA (1987) Cosmid vectors for rapid genomic waling, restriction mapping and gene transfer. Proc Natl Acad Sci USA 84:2160–2164
Wise RP, Pring DR, Gengenbach BG (1987) Mutation to male fertility and toxin insensitivity in Texas (T)-cytoplasm maize is associated with a frameshift in a mitochondrial open reading frame. Proc Natl Acad Sci USA 84:2858–2862
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Communicated by R. Hagemann
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Fauron, C.M.R., Havlik, M., Hafezi, S. et al. Study of two different recombination events in maize cmsT-regenerated plants during reversion to fertility. Theoret. Appl. Genetics 79, 593–599 (1990). https://doi.org/10.1007/BF00226870
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DOI: https://doi.org/10.1007/BF00226870