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Fine structure and peroxidatic activity of rat blood monocytes

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Summary

Rat blood monocytes contain granules of two types, primary and secondary granules, which can be distinguished on morphological grounds. Secondary granules have a halo between the limiting membrane and the matrix; primary granules have no halo and are generally more elongate than the secondary granules. In addition, there are differences in the distribution of the diameters of these two types of granules.

The cytochemical characteristics of the peroxidatic activity of these granules were investigated by using different prefixation conditions in combination with a number of DAB media differing in pH and H2O2 concentration. Prolongation of the prefixation and increasing the pH of the incubation medium increased the staining intensity of the secondary granules and decreased the staining intensity of the primary granules. In unfixed cells peroxidatic activity could be demonstrated only in the primary granules.

It is concluded from the present findings that peroxidase occurs in primary granules and not in secondary granules, whereas catalase is present in secondary granules but probably not in primary granules.

No evidence was found for the presence of microperoxisomes in rat blood monocytes.

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References

  • Ackerman, G.A., Clark, M.A.: Ultrastructural localization of peroxidase activity in normal human bone marrow cells. Z. Zellforsch. 177, 463–475 (1971)

    Google Scholar 

  • Adams, C.W.M., Bayliss, O.B., Turner, D.R.: Phagocytes, lipid-removal and regression of atheroma. J. Path. 116, 255–240 (1975)

    Google Scholar 

  • Boyum, A.: Separation of blood leucocytes, granulocytes and lymphocytes. Tissue Antigens 4, 269–274 (1974)

    Google Scholar 

  • Breton-Gorius, J., Guichard, J.: Etude au microscope électronique de la localisation des peroxydases dans les cellules de la moelle osseuse humaine. Nouv. Rev. franc. Hémat. 9, 678–687 (1969)

    Google Scholar 

  • Breton-Gorius, J., Yves Coquin, M., Guichard, J.: Activités peroxydatiques de certain granulations des neutrophils dans deux cas de déficit congenital en myeloperoxydase. C.R. Acad. Sci. (Paris) 280, Ser.D, 1753–1757 (1975)

    Google Scholar 

  • Briggs, R.T., Karnovsky, M.L., Karnovsky, M.J.: Cytochemical demonstration of hydrogen peroxide in polymorphonuclear leukocyte phagosomes. J. Cell. Biol. 64, 254–260 (1975)

    Google Scholar 

  • Cavallo, T.: Fine structural localization of endogeneous peroxidase activity in inner medullary interstitial cells of the rat kidney. Lab. Invest. 31, 458–464 (1974)

    Google Scholar 

  • Cavallo, T.: Cytochemical localization of endogeneous peroxidase activity in renal medullary collecting tubules and papillary mucosa of the rat. Lab. Invest. 34, 223–228 (1976)

    Google Scholar 

  • Daems, W.Th., Brederoo, P.: Electron microscopical studies on the structure, phagocytic properties and peroxidatic activity of resident and exudate peritoneal macrophages in the guinea pig. Z. Zellforsch. 144, 247–297 (1973)

    Google Scholar 

  • Daems, W.Th., Koerten, H.K., Soranzo, M.R.: Differences between monocyte-derived and tissue macrophages. In: The reticulo-endothelial system in health and disease: functions and characteristics, edit. by Reichard, S.M., Escobar, M.R., Friedmann, H., pp. 27–40. New York: Plenum Publishing Corporation 1976

    Google Scholar 

  • Daems, W.Th., Wisse, E., Brederoo, P., Emeis, J.J.: Peroxidatic activity in monocytes and macrophages. In: Mononuclear phagocytes in immunity, infection and pathology, edit. by van Furth, R., pp. 57–77. London: Blackwell Scientific Publishers 1975

    Google Scholar 

  • Enomoto, T., Kitani, T.: Electron microscopic studies on peroxidase and acid phosphatase in human leukocytes in normal and leukaemic cells on the phagocytosis. Acta haemat. jap. 29, 554–570 (1966)

    Google Scholar 

  • Fahimi, H.D.: Cytochemical localization of peroxidase activity in rat hepatic microbodies (peroxisomes). J. Histochem. Cytochem. 16, 547–550 (1968)

    Google Scholar 

  • Fahimi, H.D.: Cytochemical localization of peroxidatic activity of catalase in rat hepatic microbodies (peroxisomes). J. Cell Biol. 43, 275–288 (1969)

    Google Scholar 

  • Fahimi, H.D., Gray, B.A., Herzog, V.K.: Cytochemical localization of catalase and peroxidase in sinusoidal cells of rat liver. Lab. Invest. 34, 192–201 (1976)

    Google Scholar 

  • Herzog, V., Fahimi, H.D.: Glutaraldehyde fixation: a prerequisite for demonstration of the peroxidatic activity of catalase in isolated rat liver peroxisomes and crystalline beef liver catalase. J. Cell Biol. 55, 113a (1972)

  • Herzog, V., Fahimi, H.D.: Colorimetric and Cytochemical studies for determination of optimal conditions for demonstration of catalase. In: Electron microscopy and cytochemistry, edit. by Wisse, E., Daems, W.Th., Molenaar, I., van Duijn, P., pp. 111–113. Amsterdam: North Holland Publishing Company 1973

    Google Scholar 

  • Hirsch, J.G., Fedorko, M.E.: Ultrastructure of human leukocytes after simultaneous fixation with glutaraldehyde and osmium tetroxide and “postfixation” in uranyl acetate. J. Cell Biol. 38, 615–625 (1968)

    Google Scholar 

  • Hoefsmit, E.Ch.M.: Mononuclear phagocytes, reticulum cells and dendritic cells in lymphoid tissues. In: Mononuclear phagocytes in immunity, infection and pathology, edit. by van Furth, R., pp. 129–150. Lond: Blackwell Scientific Publishers 1975

    Google Scholar 

  • Huhn, D., Schmalzl, F., Demmler, K.: Monozytenleukämie. Dtsch. med. Wschr. 96, 1594–1605 (1971)

    Google Scholar 

  • Leder, L.D.: Der Blutmonozyt. Berlin-Heidelberg-New York: Springer 1967

    Google Scholar 

  • Mandell, G.L.: Catalase Superoxide dismutase and virulence of staphylococcus aureus. In vitro and in vivo studies with emphasis on staphylococcal-leukocyte interaction. J. clin. Invest. 55, 561–566 (1975)

    Google Scholar 

  • McRipley, R.J., Sbarra, A.J.: Role of the phagocyte in host-parasite interactions. XI Relationship between stimulated oxidative metabolism and hydrogen-peroxide formation, and intracellular killing. J. Bact. 94, 1417–1424 (1967)

    Google Scholar 

  • Morikawa, S., Harada, T.: Immunohistochemical localization of catalase in mammalian tissues. J. Histochem. Cytochem. 17, 30–35 (1969)

    Google Scholar 

  • Murata, F., Spicer, S.S.: Morphologic and cytochemical studies of rabbit heterophilic leukocytes. Lab. Invest. 29, 65–72 (1973)

    Google Scholar 

  • Nichols, B.A., Bainton, D.F.: Differentiation of human monocytes in bone marrow and blood. Sequential formation of two granule populations. Lab. Invest. 29, 27–40 (1973)

    Google Scholar 

  • Nichols, B.A., Bainton, D.F.: Ultrastructure and cytochemistry of mononuclear phagocytes. In: Mononuclear phagocytes in immunity, infection and pathology, (R. van Furth, ed.), pp. 17–55. London: Blackwell Scientific Publishers 1975

    Google Scholar 

  • Nichols, B.A., Bainton, D.F., Farquhar, M.G.: Differentiation of monocytes. Origin, nature and fate of their azurophil granules. J. Cell Biol. 50, 498–515 (1971)

    Google Scholar 

  • Nishimura, E.T., Whest, G.M., Yang, H.Y.: Ultrastructural localization of peroxidatic catalase in human peripheral blood leukocytes. Lab. Invest. 34, 60–68 (1976)

    Google Scholar 

  • Novikoff, A.B., Beard, M.E., Albala, A., Sheid, B., Quintana, N., Biempica, L.: Localization of endogeneous peroxidases in animal tissues. J. Microscopie 12, 381–404 (1971)

    Google Scholar 

  • Novikoff, A.B., Novikoff, P.M., Davis, C., Quintana, N.: Study on microperoxisomes II. A cytochemical method for light and electron microscopy. J. Histochem. Cytochem. 20, 1006–1023 (1972)

    Google Scholar 

  • Novikoff, A.B., Novikoff, P.M., Davis, C., Quintana, N.: Studies on microperoxisomes V. Are microperoxisomes ubiquitous in mammalian cells? J. Histochem. Cytochem. 21, 737–755 (1973)

    Google Scholar 

  • Roels, F., Wisse, E.: Distinction cytochimique entre catalase et peroxydase. C.R. Acad. Sci. (Paris) 276, Ser. D, 391–393 (1973)

    Google Scholar 

  • Roels, F., Wisse, E., Prest, B. de, Meulen, J. van der: Cytochemical discrimination between catalases and peroxidases using diaminobenzidine. Histochem. 41, 281–312 (1975)

    Google Scholar 

  • Ryan, G.B., Spector, W.G.: Macrophage turnover in inflamed connective tissue. Proc. roy.Soc. B 175, 269–292 (1970)

    Google Scholar 

  • Schmalzl, F., Braunsteiner, H.: Zytochemische Untersuchungen zur Entwicklung der großen mononucleären Zellen des Hautfensters. Acta haemat. 38, 281–291 (1967)

    Google Scholar 

  • Spector, W.G.: The macrophage in inflammation. Ser. Haemat. III, 132–144 (1970)

    Google Scholar 

  • Venkatachalam, M.A., Fahimi, H.D.: The use of beef liver catalase as a protein tracer for electron microscopy. J. Cell Biol. 42, 480–489 (1969)

    Google Scholar 

  • Vercauteren, R.E., Roels-de Schrijver, M.P., Decleir, W.: Further studies on the specific granules of leukocytes. Enzymologia 33, 279–298 (1967)

    Google Scholar 

  • Volkman, A.: The origin and fate of the monocyte. Ser. Haemat. III, 62–92 (1970)

    Google Scholar 

  • Volkman, A., Gowans, J.L.: The origin of macrophages from bone marrow in the rat. Brit. J. exp. Path. 46, 62–72 (1965)

    Google Scholar 

  • Wetzel, B.K., The fine structure and cytochemistry of developing granulocytes with special reference to the rabbit. In: Regulation of hematopoiesis II (A.S. Gordon, ed.), pp. 769–817. New York: Meredith Corporation 1970

    Google Scholar 

  • Wisse, E.: Observations on the fine structure and peroxidase of normal rat liver Kupffer cells. J. Ultrastruct. Res. 46, 393–426 (1974)

    Google Scholar 

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Authors and Affiliations

  1. Department of Dermatology, University Hospital, Leiden, The Netherlands

    H. J. van der Rhee & C. P. M. de Winter

  2. Laboratory for Electron Microscopy, University of Leiden, The Netherlands

    W. Th. Daems

Authors
  1. H. J. van der Rhee
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  2. C. P. M. de Winter
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  3. W. Th. Daems
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Additional information

The authors wish to thank Prof. Dr. M.K. Polano, and Drs. P. Brederoo, J.G.P. Tyssen, and B.J. Vermeer for stimulating discussions and their critical reading of the manuscript. The assistance of Mrs. G.C.A.M. Spigt and of J.J. Beentjes, H.K. Koerten and L.D.C. Verschragen is also gratefully acknowledged

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van der Rhee, H.J., de Winter, C.P.M. & Daems, W.T. Fine structure and peroxidatic activity of rat blood monocytes. Cell Tissue Res. 185, 1–16 (1977). https://doi.org/10.1007/BF00226665

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