Summary
Restriction fragment analysis of mitochondrial and chloroplast DNAs from a brown anther and a petaloid cytoplasmic male-sterile (cms) line revealed unique patterns for each cms line distinct from those of normal fertile cytoplasms, but identical restriction fragments for all chloroplast DNAs. The restauration of fertility through the introduction of nuclear restorer genes had no effect on the overall mitochondrial DNA (mtDNA) structure. The genomic environment and transcription patterns of several mitochondrial genes differ between cms and normal cytoplasms, while no difference has so far been detected between cms and the corresponding fertility-restored lines in mitochondrial DNAs and mRNAs. Mitochondrial translation products analysed by in-organello synthesized proteins revealed a number of polypeptides unique to each cytoplasm. Most prominent is a 17-kDa polypeptide that is present in the brown anther cms line but not in fertile mitochondria. Synthesis of this protein was not visibly affected by fertility restauration. The different cms phenotypes in carrot are thus associated with extensive and unique mtDNA rearrangements and distinct alterations in transcription and translation patterns.
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References
Bailey-Serres J, Dixon LK, Liddell AD, Leaver CJ (1986) Nuclear-mitochondrial interactions in cytoplasmic male-sterile Sorghum. Theor Appl Genet 73:252–260
Bailey-Serres J, Hanson DK, Fox TD, Leaver CJ (1987) Mitochondrial genome rearrangement leads to extension and relocation of the cytochrome c oxidase subunit I gene in sorghum. Cell 47:567–576
Banga O, Petiet J, van Bennekom JI (1964) Genetical analysis of male-sterility in carrots, Daucus carota L. Euphytica 13:75–93
Covello PS, Gray MW (1989) RNA editing in plant mitochondria. Nature 341:662–666
Dewey RE, Levings III CS, Timothy DH (1986) Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell 44:439–449
Dewey RE, Timothy DH, Levings III CS (1987) A mitochondrial protein associated with cytoplasmic male sterility in the T cytoplasm of maize. Proc Natl Acad Sci USA 84:5347–5378
Forde BG, Leaver CJ (1980) Nuclear and cytoplasmic genes controlling synthesis of variant mitochondrial polypeptides in male-sterile maize. Proc Natl Acad Sci USA 77:418–422
Forde BG, Oliver RJ, Leaver CJ (1978) Variation in mitochondrial translation products associated with male sterile cytoplasms in maize. Proc Natl Acad Sci USA 75:3841–3845
Fox TD, Leaver CJ (1981) The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell 26:315–323
Gelvin SB, Schilperoort RA (eds) (1988) Plant molecular biology. Kluwer Academic Publ, Dordrecht
Ichikawa H, Tanno-Suenaga L, Imamura J (1989) Mitochondrial genome diversity among cultivars of Daucus carota and their wild relatives. Theor Appl Genet 77:39–43
Isaac PG, Brennicke A, Dunbar SM, Leaver CJ (1985) The mitochondrial genome of fertile maize (Zea mays L.) contains two copies of the gene encoding the alpha-subunit of the F1-ATPase. Curr Genet 10:321–328
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–688
Leaver CJ, Hack E, Forde BG (1983) Protein synthesis by isolated plant mitochondria. Methods Enzymol 97:475–486
Lippok B, Brennicke A, Wissinger B (1992) The coxII gene in carrot mitochondria contains two introns. Mol Gen Genet, in press
Matthews BF, DeBonte LR (1985) Chloroplast and mitochondrial DNAs of the carrot and its wild relatives. Plant Mol Biol Rep 3:12–16
Mehring-Lemper M (1987) Genetisch-züchterische Untersuchungen zur Schaffung von Hybridsorten bei Möhren (Daucus carota L.), Dissertation, Universität Hannover
Pingitore M, Matthews B, Bottino PJ (1989) Analysis of the mitochondrial genome of Daucus carota with male sterile and male fertile cytoplasm. J Heredity 80:143–145
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Schuster W, Brennicke A (1986) Pseudocopies of the ATPase alpha-subunit gene in Oenothera mitochondria are present on different circular molecules. Mol Gen Genet 204:29–35
Small ID, Isaac PG, Leaver CJ (1987) Stoichiometric differences in DNA molecules containing the atpA gene suggest mechanisms for the generation of mitochondrial genome diversity in maize. EMBO J 6:865–869
Small ID, Suffolk R, Leaver CJ (1989) Evolution of plant mitochondrial genomes via sub-stoichiometric intermediates. Cell 58:69–76
Thompson DJ (1961) Studies on the inheritance of male sterility in the carrot, Daucus carota L. var ‘sativa’. Proc Am Soc Hortic Sci 78:332–342
Timin NI (1979) Features of the segregation of hybrid progenies of carrot for cytoplasmic male sterility (cms). Dokl Mosk Skh Akad 266:93–98
Turano FJ, DeBonte LR, Wilson KG, Matthews BF (1987) Cytochrome oxidase subunit II gene from carrot contains an intron. Plant Physiol 84:1064–1079
Wissinger B, Hiesel R, Schuster W, Brennicke A (1989) The NADH-dehydrogenase subunit 5 gene in Oenothera mitochondria contains two introns and is cotranscribed with the 18S-5S rRNA genes. Mol Gen Genet 212:56–65
Wissinger B, Schuster W, Brennicke A (1991) Trans splicing in Oenothera mitochondria: nad1 mRNAs are edited in exon and trans splicing group II intron sequences. Cell 65:473–482
Young EG, Hanson MR (1987) A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated. Cell 50:41–49
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Scheike, R., Gerold, E., Brennicke, A. et al. Unique patterns of mitochondrial genes, transcripts and proteins in different male-sterile cytoplasms of Daucus carota . Theoret. Appl. Genetics 83, 419–427 (1992). https://doi.org/10.1007/BF00226529
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DOI: https://doi.org/10.1007/BF00226529