Summary
To obtain specific immunological probes for investigation of the cellular and molecular aspects of the subcommissural organ (SCO), we produced monoclonal antibodies directed against extracts from the bovine SCO. An hybridoma cell line (C1A8B8) was isolated by screening the culture media by means of the immunofluorescence method. This clone produces an IgG1 that recognizes the ventricular secretory material of the SCO including Reissner's fiber. A competition test using C1B8A8 immunoglobulin and lectins (concanavalin A and wheat-germ agglutinin) was applied to demonstrate that both the immature and mature forms of the glycoprotein were recognized. This antibody will offer a good tool for immunocytochemical localization and immunoaffinity purification of the antigen and for isolation of cDNA clones encoding it.
Similar content being viewed by others
References
Didier M, Harandi M, Aguera M, Bancel B, Tardy M, Fages C, Calas A, Stagaard M, Møllgard K, Belin MF (1986) Differential immunocytochemical staining for glial fibrillary acidic (GFA) protein, S-100 protein and glutamine synthetase in the rat subcommissural organ, nonspecialized ventricular ependyma and adjacent neuropil. Cell Tissue Res 245:343–351
Galfre G, Howe SC, Milstein C, Butcher GW, Howard JC (1977) Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature, London, 266:550–552
Hofer H, Meinel W, Erhard H (1980) Electron microscopic study of the origin and formation of Reissner's fiber in the subcommissural organ of Cebus apella (Primates, Platyrrhina). CellTissue Res 205:295–301
Leonhardt H (1980) Ependym und Circumventriculäre Organe. In: Oksche A, Vollrath L (eds) Neuroglia I. Handbuch der mikroskopischen Anatomie des Menschen, Band IV, 10. Teil. Springer, Berlin Heidelberg New York, pp 177–665
Littlefield JW (1964) Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145:709–710
Lösecke W, Naumann W, Sterba G (1984) Preparation and discharge of secretion in the subcommissural organ of the rat. An electron-microscopic immunocytochemical study. Cell Tissue Res 235:201–206
Lösecke W, Naumann W, Sterba G (1986) Immuno-electron-microscopic analysis of the basal route of secretion in the subcommissural organ of the rabbit. Cell Tissue Res 244:449–456
Meiniel R, Meiniel A (1985) Analysis of the secretions of the subcommissural organs of several vertebrate species by use of fluorescent lectins. Cell Tissue Res 239:359–364
Meiniel A, Meiniel R (1987) Lectins as tools to study the glycoprotein secretions of the subcommissural organ. Wiss Z Karl-Marx-Univ Leipzig, Math-Naturwiss R 36:21–23
Meiniel A, Molat JL, Meiniel R (1988) Complex-type glycoproteins synthesized in the subcommissural organ of mammals. Ligh- tand electron-microscopic investigations by use of lectins. Cell Tissue Res 253:383–395
Oksche A (1969) The subcommissural organ. J Neuro-Visc Rel (Suppl) 9:111–139
Ouchterlony O (1967) Immunodiffusion and immunoelectrophoresis. In: Handbook of experimental immunology. Weir DM (ed) Blackwell scientific publications, Oxford and Edinburgh, pp 655–706
Rodríguez EM, Oksche A, Hein S, Rodríguez S, Yulis R (1984a) Comparative immunocytochemical study of the subcommissural organ. Cell Tissue Res 237:427–441
Rodríguez EM, Oksche A, Hein S, Rodríguez S, Yulis R (1984b) Spatial and structural interrelationships between secretory cells of the subcommissural organ and blood vessels. An immunocytochemical study. Cell Tissue Res 237:443–449
Rodríguez EM, Herrera H, Peruzzo B, Rodríguez S, Hein S, Oksche A (1986) Light- and electron-microscopic immunocytochemistry and lectin histochemistry of the subcommissural organ: Evidence for processing of the secretory material. Cell Tissue Res 243:545–559
Spitz M, Spitz L, Thorpe R, Eugui E (1984) Intrasplenic primary immunization for the production of monoclonal antibodies. J Immunol Methods 70:39–43
Sterba G, Kleim I, Naumann W, Petter H (1981) Immunocytochemical investigation of the subcommissural organ in the rat. Cell Tissue Res 218:659–662
Sterba G, Kiessig Chr, Naumann W, Petter H, Kleim I (1982) The secretion of the subcommissural organ. A comparative immunocytochemical investigation. Cell Tissue Res 226:427–439
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Meiniel, R., Duchier, N. & Meiniel, A. Monoclonal antibody C1B8A8 recognizes a ventricular secretory product elaborated in the bovine subcommissural organ. Cell Tissue Res. 254, 611–615 (1988). https://doi.org/10.1007/BF00226511
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00226511