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Effects of Mg2+ and adenine nucleotides on thymidylate synthetase from different mouse tumors

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Summary

Magnesium ions variably influenced activity of highly purified thymidylate synthetase preparations from different mouse tumors, activating the enzyme from Ehrlich ascites carcinoma (EAC) cells and inhibiting the enzyme from L1210 and L5178Y cells and from 5-fluorodeoxyuridine (FdUrd)-resistant EAC cells. In the presence of Mg2+ in a concentration resulting in either maximum activation or inhibition (25–30 mM) the enzymes from both the sensitive and FdUrd-resistant EAC lines and L5178Y cells were activated by ATP. Under the same conditions of Mg2+ concentration ADP and AMP inhibited the enzyme from the parental but not from the FdUrd-resistant EAC cells.

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Authors and Affiliations

  1. Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093, Warszawa, Poland

    Wojciech Rode

  2. Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, CT, USA

    Małgorzata M. Jastreboff

Authors
  1. Wojciech Rode
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  2. Małgorzata M. Jastreboff
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Rode, W., Jastreboff, M.M. Effects of Mg2+ and adenine nucleotides on thymidylate synthetase from different mouse tumors. Mol Cell Biochem 60, 73–76 (1984). https://doi.org/10.1007/BF00226300

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  • DOI: https://doi.org/10.1007/BF00226300

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