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Use of simultaneous analysis of gas-exchange and chlorophyll fluorescence quenching for analysing the effects of water stress on photosynthesis in apple leaves

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Summary

A convenient system for the rapid simultaneous measurement of both chlorophyll fluorescence quenching using a modulated light system, and of CO2, and water vapour exchange by leaves is described. The system was used in a study of the effects of water deficits on the photosynthesis by apple leaves (Malus x domestica Borkh.). Apple leaves were found to have low values of steady-state variable fluorescence, and the existence of significant fluorescence with open traps (Fo) quenching necessitated the measurement and use of a corrected Fo in the calculation of quenching components. Long-term water stress had a marked effect on both gas-exchange and chlorophyll fluorescence quenching. Non-photochemical quenching (qn) in particular was increased in water-stressed leaves, and it was particularly sensitive to incident radiation in such leaves. In contrast, rapid dehydration only affected gas exchange. Relaxation of qn quenching in the dark was slow, taking approximately 10 min for a 50% recovery, in well-watered and in draughted plants, and whether or not the plants had been exposed to high light.

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References

  • Baker NR, Horton P (1987) Chlorophyll fluorescence quenching during photoinhibition. In: Kyle DJ, Osmond CB, Amtzen CJ (eds) Photoinhibition. Elsevier, Amsterdam, pp 145–168

    Google Scholar 

  • Bilger W, Schreiber U (1986) Energy-dependent quenching of darklevelchlorophyll fluorescence in intact leaves. Photosynth Res 10: 303–308

    Google Scholar 

  • Bradbury M, Baker NR (1981) Analysis of the slow phase of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of photosystem II electron acceptors and fluorescence. Biochim Biophys Acta 635: 542–551

    Google Scholar 

  • Conroy JP, Virgona JM, Smillie RM, Barlow EWR (1988) Influence of drought acclimatisation and CO2 enrichment on osmotic adjustment and chlorophyll a fluorescence of sunflower during drought. Plant Physiol 86: 1108–1115

    Google Scholar 

  • Demmig B, Björkman O (1987) Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photon yield of O2 evolution in leaves of higher plants. Planta 171: 171–184

    CAS  Google Scholar 

  • Demmig B, Winter K, Krüger A, Czygan F-C (1988) Zeaxanthin and the heat dissipation of excess light energy in Nerium oleander exposed to a combination of highlight and water stress. Plant Physiol 87: 17–24

    Google Scholar 

  • Fanjul L, Jones HG, Treharne KJ (1981) A portable system for simultaneous measurements of transpiration and CO2 exchange. Photosynth Res 1: 83–92

    Google Scholar 

  • Ford EM (1971) How “mini-trees” could assist in nutritional studies. Report for East Mailing Research Station for 1970, pp 89–90

  • Genty B, Briantais J-M, Viera da Silva JB (1987) Effects of drought on primary photosynthetic processes of cotton leaves. Plant Physiol 83: 360–364

    Google Scholar 

  • Genty B, Briantais J-M, Baker NR (1989) Relationship between the quantum yield of photosynthetic electron transport and the quenching of chlorophyll fluorescence. Biochim Biophys Acta 990: 87–92

    CAS  Google Scholar 

  • Govindjee Downton WJS, Fork DC, Armond PA (1981) Chlorophyll-a fluorescence transient as an indicator of water potential in leaves. Plant Sci Lett 20: 191–194

    Google Scholar 

  • Havaux M, Larmoye R (1983) Chlorophyll fluorescence induction: a sensitive indicator of water stress in maize plants. Irrigation Sci 4: 147–151

    Google Scholar 

  • Horton P, Hague A (1988) Studies on the induction of chlorophyll fluorescence in isolated barley protoplasts. IV. Resolution of nonphotochemical quenching. Biochim Biophys Acta 932: 107–115

    Google Scholar 

  • Ireland CR, Long SP, Baker NR (1984) The relationship between carbon dioxide fixation and chlorophyll-a fluorescence during induction of photosynthesis at different temperatures and carbon dioxide concentrations. Planta 160: 550–558

    Google Scholar 

  • Jones HG (1983) Plants and microclimate. Cambridge University Press, Cambridge

    Google Scholar 

  • Jones HG (1985) Partitioning stomatal and non-stomatal limitations to photosynthesis. Plant Cell Environ 8: 95–104

    CAS  PubMed  Google Scholar 

  • Jones HG, Cumming IG (1984) Variation of leaf conductance and leaf water potential in apple orchards. J Hort Sci 59: 329–336

    Google Scholar 

  • Jones HG, Fanjul L (1983) Effects of water stress on CO2 exchange in apple. In: Marcelle R, Clijsters H, Poucke M van (eds) Effects of stress on photosynthesis. Martinus Nijhoff/Dr W. Junk, The Hague

    Google Scholar 

  • Krause GH, Vernotte C, Briantais JM (1982) Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Resolution into two components. Biochim Biophys Acta 679: 116–124

    CAS  PubMed  Google Scholar 

  • Lavorel J, Etienne A-L (1977) In vivo chlorophyll fluorescence. In: Primary processes of photosynthesis Barber J (ed) Elsevier/North-Holland, Amsterdam, pp 203–267

    Google Scholar 

  • Ögren E, Baker NR (1985) Evaluation of a technique for the measurement of chlorophyll fluorescence from leaves exposed to continuous white light. Plant Cell Environ 8: 539–547

    Google Scholar 

  • Oxborough K, Horton P (1986) Characterisation of the effects of Antimycin A upon high energy state quenching of chlorophyll fluorescence (qE) in spinach and pea chloroplasts. Photosynth Res 119–128

  • Peterson RB, Sivak MN, Walker DA (1987) An apparatus for the recording of CO2 induced transients in photosynthesis and fluorescence. Annual Report of Research Institute for Photosynthesis, University of Sheffield

  • Quick WP, Horton P (1984) Studies on the induction of chlorophyll fluorescence in barley protoplasts. II. Resolution of fluorescence quenching by redox state and the transthylakoid pH gradient. Proc R Soc Lond [Biol] 220: 371–382

    Google Scholar 

  • Schreiber U, Bilger W (1987) Rapid assessment of stress effects on plant leaves by chlorophyll fluorescence measurements. In: Tenhunen JD, Catarina FM, Lange OL, Oechel WC (eds) Plant response to stress. Springer, Berlin Heidelberg New York, pp; 27–53

    Google Scholar 

  • Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10: 51–62

    Google Scholar 

  • Sharkey TD (1985) Steady-state room temperature fluorescence and CO2 assimilation rates in intact leaves. Photosynth Res 7: 163–174

    Google Scholar 

  • Sicher RC, Sundblad L-G, Öquist G (1988) Effects of low temperature acclimatisation upon photosynthetic induction in barley primary leaves. Physiol Plant 73: 206–210

    Google Scholar 

  • Walker DA, Osmond LB (1986) Measurement of photosynthesis in vivo with a leaf disc electrode: correlations between light dependence of steady-state photosynthetic O2 evolution and chlorophyll-a fluorescence transients. Proc R Soc Lond [Biol] 227: 267–280

    Google Scholar 

  • Walker DA, Sivak MN, Prinsley RT, Cheesbrough JK (1983) Simultaneous measurement of oscillations in oxygen evolution and chlorophyll-a fluorescence in leaf pieces. Plant Physiol 73: 534–541

    Google Scholar 

  • Weis E, Berry JA (1987) Quantum efficiency of photosystem II in rotation to “energy”-dependent quenching of chlorophyll fluorescence. Biochim Biophys Acta 894: 198–208

    Google Scholar 

  • Wong S-C, Woo KC (1986) Simultaneous measurements of steady-state chlorophyll-a fluorescence and CO2 assimilation in leaves. Plant Physiol 80: 877–883

    Google Scholar 

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Author notes

  1. Angelo Massacci

    Present address: Istituto di Radiobiochimica ed Ecofisiologia Vegetale, CNR, via Salaria Km 29, 300, Rome, Italy

  2. Hamlyn G. Jones

    Present address: AFRC Institute of Horticultural Research, CV35 9EF, Wellesbourne, Warwick, UK

Authors and Affiliations

  1. AFRC Institute of Horticultural Research, East Mailing, Maidstone, UK

    Angelo Massacci & Hamlyn G. Jones

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  1. Angelo Massacci
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  2. Hamlyn G. Jones
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Massacci, A., Jones, H.G. Use of simultaneous analysis of gas-exchange and chlorophyll fluorescence quenching for analysing the effects of water stress on photosynthesis in apple leaves. Trees 4, 1–8 (1990). https://doi.org/10.1007/BF00226233

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  • DOI: https://doi.org/10.1007/BF00226233

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