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Inhibition of transcription by adriamycin is a consequence of the loss of negative superhelicity in DNA mediated by topoisomerase II

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Abstract

Adriamycin is commonly used as a chemotherapeutic agent and is known to intercalate into the major groove of DNA and inhibit DNA and RNA synthesis. Results presented in this communication suggest that adriamycin affects topoisomerase cleavage of DNA. The resultant change in negative superhelicity (decrease) is responsible for the decrease in transcription. This process is not dependent on the continued presence of adriamycin. The reaction between topoisomerases, DNA and adriamycin is dose-dependent. The results help to explain the relatively enhanced cytotoxicity of this drug to tumor cells.

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  1. MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, University of Stellenbosch, PO Box 63, 7505, Tygerberg, South Africa

    Marieze Tarr & Paul D. van Helden

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  1. Marieze Tarr
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  2. Paul D. van Helden
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Tarr, M., van Helden, P.D. Inhibition of transcription by adriamycin is a consequence of the loss of negative superhelicity in DNA mediated by topoisomerase II. Mol Cell Biochem 93, 141–146 (1990). https://doi.org/10.1007/BF00226185

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  • DOI: https://doi.org/10.1007/BF00226185

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