Summary
Two receptor element alleles (vp-rcy and bz-rcy) that respond to the trans-active element (Cy) controlling Mutator activity were used to analyze the strength of trans-active signals from Cy elements derived from a Mutator active line. Evidence is presented that the Mutator population tested consists mainly of a class of weak Cy elements designated as Cy:Mu. When Cy:Mu element are present in only a few copies, the strength of the combined transposition signal is weak. It is only when these active elements have a high copy number that the overall transposition signal is sufficiently strong enough to elicit a high frequency of transposition events. This study seeks to investigate the nature of the trans-active signal from Cy:Mu elements. The implication of these results for molecular studies is discussed.
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References
Alleman M, Freeling M (1986) The Mu transposable elements of maize: evidence for transposition and copy number regulation during development. Genetics 112:107–119
Bennetzen JL (1984) Transposable element Mu1 is found in multiple copies only in Robertson's Mutator maize lines. J Mol Appl Genet 2:519–524
Bennetzen JL (1985) The regulation of Mutator function and Mu1 transposition. In: Freeling M (ed) Plant genetics: UCLA Symp Mol Cell Biol. Alan R. Liss, New York, pp343–353
Bennetzen JL, Swanson J, Taylor WC, Freeling M (1984) DNA insertion in the first intron of maize Adh1 affects message levels: Cloning of progenitor and mutant Adh1 alleles. Proc Natl Acad Sci USA 81:4125–4128
Brink RA, Nilan RN (1952) The relation between light variegated and medium variegated pericarp in maize. Genetics 37:519–544
Chandler VL, Walbot V (1986) DNA modification of a maize transposable element correlates with loss of activity. Proc Natl Acad Sci USA 83:1767–1771
Chandler VL, Rivin C, Walbot V (1985) Mutator homologous sequences in inbred and Mutator lines. J Cell Biol 9c:214
Coe EH, Neuffer MG (1977) The genetics of corn. In: Sprague GF (ed) Corn and corn improvement, American Society of Agronomy, Madison/WI, pp 111–223
McClintock B (1955) Controlled mutation in Maize. Carnegie Inst Washington Yearb 54:245–255
Peterson PA (1965) A relation between the Spm and En control systems in maize. Am Nat 99:391–398
Peterson PA (1978) Controlling elements: The induction of mutability at the A2 and C loci in maize. In: Walden DB (ed) Maize breeding and genetics. Wiley and Sons/NY pp 601–635
Peterson PA (1988) The mobile element systems of maize. In: Nelson O (ed) Plants' transposable elements. Plenum, New York, pp 43–68
Robertson DS (1978) Characterization of a Mutator system in maize. Mutat Res 51:21–28
Robertson DS (1980) The timing of Mu activity in maize. Genetics 94:969–978
Robertson DS (1983) A possible dose-dependent inactivation of Mutator (Mu) in maize. Mol Gen Genet 191:86–90
Scheffler BE, Peterson PA (1985) vp-m451, a mutable allele of vp1 that responds to Cy. Maize Genet Coop Newslett 59:4
Scheffler BE, Peterson PA (1986) Systems relationships of vp-m451, a Mutator-induced, two-element system (vp-rcy and Cy). Maize Genet Coop Newslett 60:4
Schnable PS (1986) Genetic characterization of the Cy transposable element at the Bz locus of Zea mays L. PhD dissertation, Iowa State University, Ames/IA, Microfilm DET87–03761
Schnable PS, Peterson PA (1984) bz-m805137 A bronze mutable of the Cy system. Maize Genet Coop Newslett 58:9
Schnable PS, Peterson PA (1986) Distribution of genetically active Cy elements among diverse maize lines. Maydica 31:59–81
Schnable PS, Peterson PA (1988) The mutator-related Cy transposable element of Zea mays L. behaves as a near-Mendelian factor. Genetics 120:587–596
Schnable PS, Peterson PA (1989) Genetic evidence of a relationship between two maize transposable element systems: Cy and Mutator. Mol Gen Genet 215:317–321
Schnable PS, Peterson PA, Saedler H (1989) The bz-rcy allele of the Cy transposable element system of Zea mays contains a Mu-like element insertion. Mol Gen Genet 217:459–463
Steel RGD, Torrie JH (1980) Principles and procedures of statistics, 2nd edn. McGraw-Hill, New York
Walbot V (1986) Inheritance of Mutator activity in Zea mays as assayed by somatic instability of the bz2-mul allele. Genetics 114:1293–1312
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Communicated by F. Salamini
Journal Paper No. J-13083 of the Iowa Agriculture and Home Economics Experiment Station, Ames, IA, Project No. 2381
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Scheffler, B.E., Peterson, P.A. Somatic Mutator activity expression is dependent on the strength of Cy trans-active signals in maize. Theoret. Appl. Genetics 79, 449–456 (1990). https://doi.org/10.1007/BF00226151
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DOI: https://doi.org/10.1007/BF00226151