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Anti-idiotypic antibodies as probes of cell surface receptors

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Summary

Anti-idiotypic antibodies have proven to have unique applications as probes in both functional and biochemical studies of cell surface receptors. Anti-idiotypic receptor antibodies have been prepared to antibodies which bind to purified ligand, as in the case of insulin, retinol-binding protein, the mammalian reovirus receptor, and the neutrophil chemotatic receptor, and to natural ligand analogs, such as the beta-adrenergic antagonist alprenolol. These systems have documented the usefulness of anti-idiotypic antibodies in the quantitation and modulation of specific membrane receptors on a variety of cell types. Anti-idotypic antibodies have also been utilized for the isolation of specific membrane receptors, e.g., reovirus and B-1 H globulin receptors. Some anti-idiotypic receptor antibodies, e.g., insulin and reovirus systems, have been shown to mimic the physiological properties of ligand upon binding to cellular receptors. These antibodies enable a new dimension of both receptor based cellular studies and therapeutic regimens. This review focuses on the past use of anti-idiotypic antibodies as probes of cell surface receptors, and on the methodologies required for the successful application of anti-idiotypic antibodies for use in further membrane receptor studies, and of the genes which encode and regulate these receptors. We also discuss the use of anti-idiotypic antibodies in the understanding of and therapeutic approach to receptor related diseases.

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  1. Department of Pathology, Harvard Medical School, 25 Shattuck Street, 02115, Boston, MA, USA

    Glen N. Gaulton, Man Sung Co, Hans-Dieter Royer & Mark I. Greene

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  1. Glen N. Gaulton
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  2. Man Sung Co
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Dr. Gaulton received the NRSA Viral Oncology Training Grant t# CA 09031.

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Gaulton, G.N., Co, M.S., Royer, HD. et al. Anti-idiotypic antibodies as probes of cell surface receptors. Mol Cell Biochem 65, 5–21 (1984). https://doi.org/10.1007/BF00226015

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