Summary
Highly sensitive and specific synthetic substrates were used to quantitate cathepsin B and D activity in peritoneal macrophages in response to stimulation in vivo with mineral oil and thioglycollate. After intraperitoneal instillation of mineral oil the activity of cathepsin B increased significantly (to 15 300 units/mg protein versus 7 340 in saline controls), reaching values approaching those found in alveolar macrophages (18 400 units/mg protein). Significantly greater stimulation of enzyme activity was obtained after intraperitoneal instillation of thioglycollate (23 600 units/mg protein). Cathepsin D activity also increased significantly after both mineral oil and thioglycollate. However, the increase was moderate (from 806 to about 1 200 units/mg protein), remaining still more than six times lower-than in alveolar macrophages. The data are the first to demonstrate that cathepsin B activity can be stimulated in vivo in peritoneal macrophages by instillation of agents that induce acute inflammation. They also point to a differential control of expression of cathepsin B and D activity in both peritoneal and alveolar macrophages in spite of the common lysosomal origin of the two enzymes.
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Abbreviations
- Cbz:
-
α-N-benxyloxycarbonyl
- 2NA:
-
2-naphthylamine
- EDTA:
-
ethylenediamine tetraacetate
- DMSO:
-
dimethylsulfoxide
- PBS:
-
phosphate-buffered saline
- PM:
-
peritoneal macrophage
- AM:
-
alveolar macrophage
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Lesser, M., Change, J.C. & Orlowski, M. Cathepsin B and D activity in stimulated peritoneal macrophages. Mol Cell Biochem 69, 67–73 (1985). https://doi.org/10.1007/BF00225928
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DOI: https://doi.org/10.1007/BF00225928