Abstract
To obtain new information on phylogenetic relationships between wild and cultivated broad bean, restriction fragment length polymorphism (RFLP) analysis of chloroplast (cp) DNAs from Vicia faba and eight subspecies/species of its close wild relatives grouped together in the Narbonensis complex was carried out using 14 restriction endonucleases. The molecular sizes of the cpDNAs obtained were similar (122.6–123.4 kbp), indicating that they had all lost one of inverted repeats. Among the more than 300 sites surveyed, the three subspecies within V. narbonensis, which exhibit just as many types of karyotypes, were shown to have identical cp fragment patterns. Genetic distances between all of the pairs of species were calculated from RFLP data. The cpDNA diversity within the Narbonensis complex was found to be more extensive than expected, except for the genetic relationship between V. hyaeniscyamus and V. johannis in which a total of three mutations were detected among the 300 sites sampled, thereby showing their close relatedness. The cpDNA of V. faba vis-a-vis its wild relatives also exhibited startling differences, indicating a clear division of Vicia species into two distinct lineages. This analysis unambiguously provides new evidence that the wild species grouped in the complex did not contribute their plastomes to the evolution of V. faba, and hence none of the species can be considered to be putative allies of broad bean. The present study also demonstrates profound cpDNA diversity among closely related species that have lost one of inverted repeats.
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Ball PW (1968) Vicia L. In: Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, Webb DA (eds) Flora Europaea 2. Cambridge University Press, London, pp 129–136
Birch ANE, Tithecott MT, Bisby FA (1985) Vicia johannis and wild relatives of the faba bean: a taxonomic study. Econ Bot 39:177–190
Cubero JI (1984) Taxonomy, distribution and evolution of the Faba bean and its wild relatives. In: Witcombe JR, Erskine W (eds) Genetic resources and their exploitation-chickpeas, Faba beans and lentils. Martinus Nijhoff/Dr W Junk, The Netherlands, pp 131–143
Duke JA (1981) Handbook of leumes of world economic importance. Plenum Press, New York
Hanelt P, Mettin D (1989) Biosystematics of the genus Vicia L. (Leguminosae). Annu Rev Ecol Syst 20:199–223
Kay DE (1979) Food legumes. TPI crop and product digest, no. 3, New York
Ko K, Straus NA, Williams JP (1983) Mapping the chloroplast DNA of Vicia faba. Curr Genet 7:255–263
Ko K, Orfanides AG, Straus NA (1987) A model for the evolution of the Vicia faba chloroplast genome. Theor Appl Genet 74:125–139
Koller B, Delius H (1980) Vicia faba chloroplast DNA has only one set of ribosomal RNA genes as shown by partial denaturation mapping and R-Loop analysis. Mol Gen Genet 178:261–269
Ladizinsky G (1975) Seed protein electrophoresis of the wild and cultivated species of section Faba of Vicia. Euphytica 24:785–788
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Press, Cold Spring Harbor, N.Y.
Maxted N, Khattab AMA, Bisby FA (1991) The newly discovered relatives of Vicia faba L. do little to resolve the enigma of its origin. Bot Chron 10:435–465
Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
Ogihara Y, Tsunewaki K (1982) Molecular basis of the genetic diversity of cytoplasm in Triticum and Aegilops. I. Diversity of the chloroplast genome and its lineage revealed by the restriction pattern of ct-DNAs. Jpn J Genet 57:371–396
Ogihara Y, Tsunewaki K (1988) Diversity and evolution of chloroplast DNA in Triticum and Aegilops as revealed by restriction fragment analysis. Theor Appl Genet 76:321–332
Ogihara Y, Terachi T, Sasakuma T (1992) Structural analysis of length mutations in a hot-spot region of wheat chloroplast DNAs. Curr Genet 22:251–258
Pal M, Khoshoo TN (1973) Evolution and improvement of cultivated Amaranthus. 7. Cytogenetic relationships in vegetable Amaranthus. Theor Appl Genet 43:343–350
Palmer JD (1985) Chloroplast DNA and molecular phytogeny. Biol Essays 2:263–267
Palmer JD (1986) Chloroplast DNA and phylogenetic relationships. In: Dutta SK (ed) DNA systematics, vol II. Plants. CRC press, Boca Rouge, Fla., pp 63–80
Palmer JD, Thompson WF (1982) Chloroplast DNA rearrangements are more frequent when a large inverted repeat sequence is lost. Cell 29:537–550
Palmer JD, Zamir D (1992) Chloroplast evolution and phylogenetic relationships in Lycopersicon. Proc Natl Acad Sci USA 79:5006–5010
Palmer JD, Jorgensen RA, Thompson WF (1985) Chloroplast DNA variation and evolution in Pisum: patterns of change and phylogenetic analysis. Genetics 109:195–213
Palmer JD, Jansen RK, Michaels HJ, Chase MW, Manhart JR (1988) Chloroplast DNA variation and plant phytogeny. Ann Mo Bot Gard 75:1180–1206
Perrino P, Maruca G, Linsalata V, Bianco VV, Lester RN, Lattanzio V (1989) Flavonoid taxonomic analysis of Vicia species of section Faba. Can J Bot 67:3529–3533
Plitmann U (1967) Biosystematical study in the annual species of Vicia of the Middle East. The Hebrew University of Jerusalem Press, Jerusalem
Pradhan AK, Prakash S, Mukhopadhyay A, Pental D (1992) Phytogeny of Brassica and allied genera based on variation in chloroplast and mitochondrial DNA patterns: molecular and taxonomic classifications are incongruous. Theor Appl Genet 85:331–340
Raina SN (1990) Genome organization and evolution in the genus Vicia. In: Kawano S (ed) Biological approaches and evolutionary trends in plants. Academic Press, London, pp 183–201
Raina SN, Bisht MS (1988) DNA amounts and chromatin compactness in Vicia. Genetica 77:65–77
Raina SN, Rees H (1983) DNA variation between and within chromosome complements of Vicia species. Heredity 51:335–346
Raina SN, Yamamoto K, Murakami M (1989) Intraspecific hybridization and its bearing on chromosome evolution in Vicia narbonensis (Fabaceae). Plant Syst Evol 167:201–217
Schäfer HI (1973) Zur Taxonomie der Vicia narbonensis Gruppe. Kulturpflanz 21:211–273
Sokal RR, Sneath PHA (1963) Principles of numerical taxonomy. Freeman & Co, San Francisco
Summerfield RJ, Bunting AH (1980) Advances in legume science. Royal Botanical Garden, Kew, UK
Sytsma KJ, Gottlieb LD (1986) Chloroplast DNA evolution and phylogenetic relationships in Clarkia Sect. Peripetasma (Onagraceae) Evolution 40:1248–1261
Torres AM, Weeden NF, Martin A (1993) Linkage among isozyme, RFLP and RAPD markers in Vicia faba. Thoer Appl Genet 85:937–945
van de Ven WTG, Powell M, Ramsay G, Waugh R (1990) Restriction fragment length polymorphisms as genetic markers in Vicia. Heredity 65:329–342
Zohary D, Hopf M (1973) Domestication of pulses in the old world. Science 182:887–894
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Raina, S.N., Ogihara, Y. Chloroplast DNA diversity in Vicia faba and its close wild relatives: implications for reassessment. Theoret. Appl. Genetics 88, 261–266 (1994). https://doi.org/10.1007/BF00225907
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DOI: https://doi.org/10.1007/BF00225907