Abstract
In order to assess fluid domains in the genome of Dasypyrum villosum, Feulgen/DNA cytophotometric determinations and molecular and cytological DNA-DNA hybridization experiments were carried out in resting embryos and developing seedlings from yellow and brown caryopses belonging to different populations. The cytophotometric data showed that the basic amount of nuclear DNA is, on average, 12% higher in 2-day-old seedlings from yellow caryopses as compared to those from brown caryopses. It increases in each individual during seed germination, to a higher extent in seedlings from yellow caryopses than in those from brown caryopses. DNA content also differs up to 13% between plants within a caryopsis-colour group and up to 40% between populations. Dot-blot hybridization of a 396-bp D. villosum-specific DNA repeat to genomic DNA extracted from embryos in dry seeds, or from seedlings belonging to single progenies of plants from different populations, confirmed the cytophotometric results. The redundancy in the genome of sequences hybridizing to the 396-bp element differs significantly both between populations and between plant progenies within a population. During seed germination these sequences are the more amplified the less they are redundant in the genome of resting embryos, and amplification occurs to a significantly-greater extent in seedlings from yellow caryopses than in those from brown caryopses. 3H-labelled 396-bp sequences hybridize at or near the telomeres of most chromsome pairs though only to the shorter of the two subtelocentric pairs. The hybridization level is higher in seedlings from yellow caryopses that in those from brown caryopses, and a linear correlation exists between the number of silver grains counted over the labelled regions of each chromosome pair in the two groups of seedlings. Possible control mechanisms of the observed changes in the nuclear genome, and the role of these changes in developmental pregulation and environmental adaptation, are discussed.
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References
Appels R, Dennis ES, Smyth DR, Peacock WJ (1981) The repeated DNA sequences from Mu heterochromatic regions of rye (Secale cereale) chromosomes. Chromosoma 84:265–277
Bassi P (1990) Quantitative variations of nuclear DNA during plant development: a critical analysis. Biol Rev 65:185–225
Baum BR (1983) A phylogenetic analysis of the tribe Triticeae (Poaceae) based on morphological characters of the genera. Can J Bot 61:518–535
Bennett MD (1987) Variation in genomic form in plants and its ecological implications. New Phytol 106:177–200
Bennett MD, Smith JB (1976) Nuclear DNA amounts in angiosperms. Phil Trans R Soc Lond, B 274:227–274
Bucholl M, Buchowicz J (1992) Synthesis of extrachromosomal DNA and telomere-related sequences in germinating wheat embryos. Seed Sci Res 2:141–146
Cavallini A, Natali L (1991) Intraspecific variation of nuclear DNA content in plant species. Caryologia 44:93–107
Cavallini A, Natali L, Cionini G, Gennai D (1993) Nuclear DNA variability within Pisum sativum (Leguminosae): nucleotypic effects on plant growth. Heredity 70:561–565
Ceccarelli M, Cionini PG (1993) Temperatura ambientale e fluidità quantitativa del genoma nucleare di Festuca arundinacea Schreber. Giorn Bot Ital 127:557
Ceccarelli M, Falistocco E, Cionini PG (1992) Variation of genome size and organization within hexaploid Festuca arundinacea. Theor Appl Genet 83:273–278
Ceccarelli M, Minnelli S, Falcinelli M, Cionini PG (1993) Genome size and plant development in hexaploid Festuca arundinacea. Heredity 71:555–560
Chen D, Osborne DJ (1970) Ribosomal genes and DNA replication in germinating wheat embryos. Nature 225:336–340
Chen EY, Seeburg PH (1985) Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4: 165–170
Cionini PG (1989) Nuclear DNA changes during plant development. Giorn Bot Ital 123:111–121
Cionini PG, Bassi P, Cremonini R, Cavallini A (1985) Cytological localization of fast-renaturing and satellite DNA sequences in Vicia faba. Protoplasma 124:106–111
Cremonini R, Colonna N, Stefani A, Galasso I, Pignone D (1994) Nuclear DNA content, chromatin organization and chromosome banding in Dasypyrum villosum (L.) P. Candargy. Heredity (in press)
Cullis CA (1990) DNA rearrangements in reponse to environmental stress. Adv Genet 28:73–97
De Gara L, Paciolla C, Liso R, Stefani A, Arrigoni O (1991) Correlation between ascorbate peroxidase activity and some anomalies of seedlings from aged caryopses of Dasypyrum villosum (L.) Borb. J Plant Physiol 137:697–700
Della Porta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21
De Pace C, Paolini R, Scarascia Mugnozza GT, Qualset CO, Delre V (1990) Evaluation and utilization of Dasypyrum villosum as a genetic resource for wheat improvement. In: Srivastava JP, Damania AB (eds) Wheat genetic resources: meeting diverse needs. Wiley and Sons, Chichester, pp 279–289
De Pace C, Delre V, Scarascia Mugnozza GT, Qualset CO, Cremonini R, Frediani M, Cionini PG (1992) Molecular and chromosomal characterization of repeated and single-copy DNA sequences in the genome of Dasypyrum villosum. Hereditas 116:55–65
Dvořák J (1983) Evolution of multigene families: the ribosomal RNA loci of wheat and related species. In: Brown HD, Clegg MT, Kahler AL, Weir BS (eds) Plant population genetics, breeding, and genetic resources. Sinauer Ass. Inc., Sunderland, Massachusset, pp 83–97
Hennen S, Mizuno S, MacGregor HC (1975) In-situ hybridization of ribosomal DNA labelled with 125iodine to metaphase and lampbrush chromosomes from newts. Chromosoma 50:349–369
Innocenti AM, Bitonti MB (1980) Differente invecchiamento nelle cariossidi ‘chiare’ e ‘scure’ di Haynaldia villosa Schur. Uno studio citofotometrico nei meristemi radicali quiescenti. Giorn Bot Ital 114:29–35
Innocenti AM, Bitonti MB (1983) Different duration of the mitotic cycle in seedlings from brown and black caryopses of Haynaldia villosa Schur. Caryologia 36:27–32
Kikuki S, Takaiwa F, Oono K (1987) Variable copy number of DNA sequences in rice. Mol Gen Genet 210:373–380
MacGregor HC, Mizuno S (1976) In-situ hybridization of ‘nicktranslated’ 3H-ribosomal DNA to chromosomes from salamanders. Chromosoma 89:1–7
Martin J, Hesemann CU (1988) Cytogenetic investigations in wheat, rye and triticale. I. Evaluation of improved Giemsa, Cand fluorochrome-banding techniques in rye chromosomes. Heredity 61:459–467
Nagl W (1990) Gene amplification and related events. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Springer, Berlin Heidelberg New York, 11:153–201
Natali L, Cavallini A, Cionini G, Sassoli O, Cionini PG, Durante M (1993) Nuclear DNA changes within Helianthus annus L.: changes within single progenies and their relationships with plant development. Theor Appl Genet 85:506–512
Rigby WJ, Dieckmann M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nicktranslation with DNA polymerase I. J Mol Biol 113:237–251
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chainterminating inhibitors. Proc Natl Acad Sci USA 4:165–170
Seguy E (1936) Code universal des coleurs. Lechevalier, Paris
Stefani A, Onnis A (1984) Significato ecologico della dormienza nelle cariossidi ‘normali’ e ‘scure’ di Dasypyrum villosum (L.) P. Candargy. Inf Bot Ital 16:103–112
Walbot V, Cullis CA (1985) Rapid genomic change in higher plants. Annu Rev Plant Physiol 36:367–396
Zheng KL, Castiglione S, Biasini MG, Biroli A, Morandi C, Sala F (1987) Nuclear DNA amplification in cultured cells of Oryza sativa L.. Theor Appl Genet 74:65–70
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Frediani, M., Colonna, N., Cremonini, R. et al. Redundancy modulation of nuclear DNA sequences in Dasypyrum villosum . Theoret. Appl. Genetics 88, 167–174 (1994). https://doi.org/10.1007/BF00225893
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DOI: https://doi.org/10.1007/BF00225893