Evidence that quantal Ca²⁺ release in HSG cells is not due to ‘all-or-none’ release from discrete Ca²⁺ stores with differing sensitivities to IP₃

Abstract

We demonstrate that the application of the muscarinic agonist carbachol to the human salivary epithelial cell line HSG elicits the now well-known phenomenon of ‘quantal’ Ca²⁺-release; namely, that the application of a submaximal concentration of agonist results in the release of only a portion of the agonist-sensitive intracellular Ca²⁺ pool. One explanation that has been proposed to account for this effect is that there are multiple intracellular Ca²⁺ stores, each with a different agonist sensitivity, which release Ca²⁺ in an ‘all-or-none’ fashion. We test this hypothesis in intact HSG cells with an experimental protocol designed to preferentially load less-agonist-sensitive stores with ⁴⁵Ca²⁺ and more-agonist-sensitive stores with ⁴⁵Ca²⁺. However, contrary to the expectations of the above explanation, these cells do not preferentially release ⁴⁵Ca²⁺ in response to low concentrations of agonist. Thus our data suggest that quantal Ca²⁺ release must arise from some other property of the stores or their Ca²⁺ release channels.