Abstract
Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA.
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Ding, X.Z., Smallridge, R.C., Galloway, R.J. et al. Rapid assay of HSF1 and HSF2 gene expression by RT-PCR. Mol Cell Biochem 158, 189–192 (1996). https://doi.org/10.1007/BF00225845
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DOI: https://doi.org/10.1007/BF00225845