Summary
Utilizing the unlabeled antibody enzyme method, we investigated the distribution of hypothalamic elements immunoreactive with antibodies to growth hormonerelease inhibiting hormone (GH-RIH). Immunostained elements, resembling neural processes, are distributed along a pathway corresponding to a portion of the tuberoinfundibular tract. However, GH-RIH fibers are caudal, dorsal and medial to LH-RH fibers detected by the same technique. Similar topographic arrangements are noted in coronal and sagittal sections. Comparable results were obtained with two different preparations of antisera to GH-RIH. No cell bodies specifically stained by anti-GH-RIH were detected. Our data agree with those of other investigators using immunohistochemical techniques.
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This investigation was supported by grants: 1) NIH (7-F02-HD-05106-01) Postdoctoral fellowship to J.C.K., 2) NIH (R01-HD-00867-12A1) to A.A.G. and 3) NIH (R01-AM-09094-9) to A.A.
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King, J.C., Gerall, A.A., Fishback, J.B. et al. Growth hormone-release inhibiting hormone (GH-RIH) pathway of the rat hypothalamus revealed by the unlabeled antibody peroxidase-antiperoxidase method. Cell Tissue Res. 160, 423–430 (1975). https://doi.org/10.1007/BF00225761
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DOI: https://doi.org/10.1007/BF00225761