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Repeated DNA sequences isolated by microdissection. II. Comparative analysis in Hordeum vulgare and Triticum aestivum

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Abstract

The genomic organization of two satellite DNA sequences, pHvMWG2314 and pHvMWG2315, of barley (Hordeum vulgare, 2n=14, HH) was studied by comparative in situ hybridization (ISH) and PCR analysis. Both sequences are members of different RsaI families. The sequence pHvMWG2314 is a new satellite element with a monomer unit of 73 bp which is moderately amplified in different grasses and occurs in interstitial clusters on D-genome chromosomes of hexaploid wheat (Triticum aestivum, 2n=42, AABBDD). The 331-bp monomer pHvMWG2315 belongs to a tandemly amplified repetitive sequence family that is present in the Poaceae and preferentially amplified in Aegilops squarrosa (2n=14, DD), H. vulgare and Agropyron elongatum. (2n=14, EE). The first described representative of this family was pAs 1 from Ae. squarrosa. Different sequences of one satellite DNA family were amplified from Ae. squarrosa, A. elongatum and H. vulgare using PCR. Characteristic differences between members of the D and H genome occurred in a variable region which is flanked by two conserved segments. The heterogeneity within this element was exploited for the cytogenetic analysis of Triticeae genomes and chromosomes. Comparative ISH with pHvMWG2315 identified individual wheat and barley chromosomes under low (75%) and high (85%) hybridization stringency in homologous and heterologous systems. We propose the designation Tas330 for the Triticeae amplified sequence (Tas) satellite family with a 330 bp average monomer length.

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References

  • Anamthawat-Jónsson K, Heslop-Harrison, JS (1993) Isolation and characterization of genome-specific DNA sequences in Triticeae species. Mol Gen Genet 240:151–158

    Google Scholar 

  • Appels R, Driscoll C, Peacock WJ (1978) Heterochromatin and highly repeated DNA sequences in rye (Secale cereale). Chromosoma 70:67–89

    Google Scholar 

  • Appels R, Reddy P, McIntyre CL, Moran LB, Frankel OH, Clarke BC (1989) The molecular-cytogenetic analysis of grasses and its application to studying relationships among species of the Triticeae. Genome 31:122–133

    Google Scholar 

  • Appels R, Gerlach WL, Dennis ES, Swift H, Peacock WJ (1980) Molecular and chromosomal organization of DNA sequences coding for the ribosomal RNAs in cereals. Chromosoma 78:293–311

    Google Scholar 

  • Bedbrook JR, Jones J, O'Dell M, Thompson RD, Flavell RB (1980) A molecular description of telomeric heterochromatin in Secale species. Cell 19:545–560

    Article  CAS  PubMed  Google Scholar 

  • Bournival B, Obanni M, Abad A, Ohm H, MacKenzie S (1994) Isolation of a new species-specific repetitive sequence from Thinopyrum elongatbcum and its use in the studies of alien translocations. Genome 37:97–104

    Google Scholar 

  • Brandes A, Röder MS, Ganal MW (1995) Barley telomeres are associated with two different types of satellite DNA sequences. Chromosome Res 3:315–320

    Google Scholar 

  • Busch W, Martin R, Herrmann RG (1994) Sensitivity enhancement of fluorescence in situ hybridization on plant chromosomes. Chromosome Res 2:15–20

    Google Scholar 

  • Busch W, Martin R, Herrmann RG, Hohmann U (1995) Repeated DNA sequences isolated by microdissection. I. Karyotyping of barley (Hordeum vulgare L.). Genome 38:1082–1090

    Google Scholar 

  • Cabrera A, Friebe B, Jiang J, Gill BS (1995) Characterization of Hordeum chilense by C-banding and in situ hybridization using highly repeated DNA probes. Genome 38:435–442

    Google Scholar 

  • Dvorak J, Zhang HB (1992) Reconstruction of the phylogeny of the genus Triticum from variation in repeated nucleotide sequences. Theor Appl Genet 84:419–429

    Google Scholar 

  • Flavell RB, O'Dell M, Jones J (1980) Cereal genome studies and plant breeding research. In: Rubenstein I, Phillips RL, Green CE (eds) Genetic engineering of crops. University of Minnesota Press, Minneapolis, Minnesota, pp. 76–90

    Google Scholar 

  • Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885

    CAS  PubMed  Google Scholar 

  • Gerlach WL, Dyer TA (1980). Sequence organization of the repeating units in the nucleus of wheat which contains 5s rRNA genes. Nucleic Acids Res 8:4851–4855

    Google Scholar 

  • Guidet F, Rogowski P, Taylor C, Song W, Langridge P (1991) Cloning and characterization of a new rye-specific repeated sequence. Genome 34:81–87

    Google Scholar 

  • Hutchinson J, Miller TE (1982) The nucleolar organizers of tetraploid and hexaploid wheats revealed by in situ hybridization. Theor Appl Genet 61:285–288

    Google Scholar 

  • Islam AKMR (1983) Ditelosomic additions of barley chromosomes to wheat. In: Sakamoto S (ed) Proc 6th Int Wheat Genet Symp, Kyoto University, Kyoto, Japan, pp. 233–238

    Google Scholar 

  • Isono K, Sasakuma T, Tsujimoto H, Gill BS (1991) Characterization of a repetitive DNA clone, pAs1, isolated from Aegilops squarrosa. In: Sasakuma T, Kinoshita T (eds) Nuclear and organellar genomes of wheat. Kihara Memorial Foundation, Japan, pp. 81–84

    Google Scholar 

  • Jelenik, WR, Schmid, CW (1982) Repetitive sequences in eucaryotic DNA and their expression. Annu Rev Biochem 51:813–844

    Article  CAS  PubMed  Google Scholar 

  • Jiang J, Gill BS (1994) New 18S·26s ribosomal RNA gene loci: chromosomal landmarks for the evolution of polyploid wheats. Chromosoma 103:179–185

    Google Scholar 

  • Jones JDG, Flavell RB (1982) The structure, amount and chromosomal localization of defined repeated DNA sequences in species of the genus Secale. Chromosoma 86:613–641

    Google Scholar 

  • Leitch IJ, Leitch AR, Heslop-Harrison JP (1991) Physical mapping of plant DNA sequences by simultaneous in situ hybridization of two differently labelled fluorescent probes. Genome 34:329–333

    Google Scholar 

  • McIntyre CL, Clarke BC, Appels R (1988) Amplification and dispersion of repeated DNA sequences in the Triticeae. Pl Syst Evol 160:39–59

    Google Scholar 

  • McIntyre CL, Pereira S, Moran LB, Appels R (1990) New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat. Genome 33:635–640

    CAS  PubMed  Google Scholar 

  • McNeil D, Lagudah ES, Hohmann U, Appels R (1994) Amplification of DNA sequences in wheat and its relatives — The Dgas44 and R350 families of repetitive sequences. Genome 37:320–327

    Google Scholar 

  • Meinkoth J, Wahl G (1984). Hybridization of nucleic acids immobilized on solid supports. Anal Biochem 138:267–284

    Google Scholar 

  • Metzlaff M, Troebner W, Baldauf F, Schlegel R, Cullum J (1986) Wheat specific repetitive DNA sequences — construction and characterization of four different genomic clones. Theor Appl Genet 72:207–210

    Google Scholar 

  • Mukai Y, Endo TR, Gill BS (1990) Physical mapping of the 5SrRNA multigene family in common wheat. J Hered 81:290–295

    Google Scholar 

  • Mukai Y, Endo TR, Gill BS (1991) Physical mapping of the 18S·26S rRNA multigene family in common wheat: Identification of a new locus. Chromosoma 100:71–78

    Google Scholar 

  • Mukai Y, Nakahara Y, Yamamoto M (1993) Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome 36:489–494

    Google Scholar 

  • Nagaki K, Tsujimoto H, Isono K, Sasakuma T (1995) Molecular characterization of a tandem repeat, Afa family, and its distribution among Triticeae. Genome 38:479–486

    Google Scholar 

  • Rayburn AL, Gill BS (1986) Isolation of a D-genome-specific repeated DNA sequence from Aegilops squarrosa. Plant Mol Biol Rep 4:102–109

    Google Scholar 

  • Rayburn AL, Gill BS (1987) Molecular analysis of the D-genome of the Triticeae. Theor Appl Genet 73:385–388

    Google Scholar 

  • Rimpau J, Smith DB, Flavell RB (1978) Sequence organization analysis of the wheat and rye genomes by interspecies DNA/DNA hybridization. J Mol Biol 123:327–359

    Google Scholar 

  • Rimpau J, Smith DB, Flavell RB (1980) Sequence organization in barley and oat chromosomes revealed by interspecies DNA/DNA hybridization. J Hered 44:131–149

    Google Scholar 

  • Röder MS, Lapitan NLV, Sorrells ME, Tanksley SD (1993) Genetic and physical mapping of barley telomeres. Mol Gen Genet 238:294–303

    PubMed  Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    CAS  PubMed  Google Scholar 

  • Svitashev S, Bryngelsson T, Vershinin A, Pedersen C, Säll T, von Bothmer R (1994) Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences. Theor Appl Genet 89:801–810

    Google Scholar 

  • The TT, Gupta RB, Dyck PL, Appels R, Hohmann U, McIntosh RA (1992) Characterization of stem rust-resistant derivatives of wheat cultivar Amigo. Euphytica 58:245–252

    Google Scholar 

  • Vershinin A, Svitashev S, Gummesson P-O, Salomon B, von Bothmer R, Bryngelsson, T (1994) Characterization of a family of repeated DNA sequences in Triticeae. Theor Appl Genet 89:217–225

    Google Scholar 

  • Wu K-S, Tanksley SD (1993) Genetic and physical mapping of telomeres and macrosatellites of rice. Plant Mol Biol 22:861–872

    Google Scholar 

  • Wu H-K, Chung M-C, Wu T, Ninc C-N, Wu R (1991) Localization of specific repetitive DNA sequences in individual rice chromosomes. Chromosoma 100:330–338

    Google Scholar 

  • Zhang H-B, Dvorak J (1990) Isolation of repeated DNA sequences from Lophopyrum elongatum for detection of Lophopyrum chromatin in wheat genomes. Genome 33:283–293

    Google Scholar 

Download references

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Authors and Affiliations

  1. Botanisches Institut der Ludwig-Maximilians-Universität München, Menzinger Straße 67, D-80638, München, Germany

    Winfried Busch, Reinhold G. Herrmann & Uwe Hohmann

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  1. Winfried Busch
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  2. Reinhold G. Herrmann
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  3. Uwe Hohmann
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Communicated by B. S. Gill

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Busch, W., Herrmann, R.G. & Hohmann, U. Repeated DNA sequences isolated by microdissection. II. Comparative analysis in Hordeum vulgare and Triticum aestivum . Theoret. Appl. Genetics 93, 164–171 (1996). https://doi.org/10.1007/BF00225741

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  • DOI: https://doi.org/10.1007/BF00225741

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