Abstract
A major gene underlying quantitative resistance of barley against Pyrenophora graminea, a seedborne pathogen causing leaf stripe, was mapped with molecular markers in a barley doubled haploid (DH) population derived from the cross ‘Proctor’ x ‘Nudinka’. This quantitative trait locus (QTL) accounts for r 2= 58.5% and was mapped on barley chromosome 1, tightly linked to the “naked” gene. A second resistance QTL accounting for 29.3% of the variation in the trait was identified on the P arm of barley chromosome 2. Another two minor QTLs were detected by further analysis. None of the QTLs was found in the barley chromosome 2 “Vada” region studied by Giese et al. (1993).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Boulif M, Wilcoxson RD (1988) Inheritance of resistance to Pyrenophora graminea in barley. Plant Dis 72:233–238
Chen FQ, Prehn D, Hayes PM, Mulrooney D, Corey A, Vivar H (1994) Mapping genes for resistance to barley stripe rust (Puccinia striiformis f. sp. hordei). Theor Appl Genet 88:215–219
Delogu G, Porta-Puglia A, Vannacci G (1989) Resistance of winter barley varieties subjected to natural inoculum of Pyrenophora graminea. J Genet Breed 43:61–66
Dirlewanger E, Isaac PG, Ranade S, Belajouza M, Cousin R, de Vienne D (1994) Restriction fragment length polymorphism analysis of loci associated with disease resistance genes and developmental traits in Pisum sativum L. Theor Appl Genet 88:17–27
Gatti A, Rizza F, Delogu G, Terzi V, Porta-Puglia A, Vannacci G (1992) Physiological and biochemical variability in a population of Drechslera graminea. J Genet Breed 46:179–186
Giese H, Holm-Jensen AG, Jensen HP, Jensen J (1993) Localization of the Laevigatum powdery mildew resistance gene to barley chromosome 2 by the use of RFLP markers. Theor Appl Genet 85:897–900
Haahr V, Skou JP, Jensen HP (1989) Inheritance of resistance to barley leaf stripe (Drechslera graminea). Vortr Pflanzenzuecht 15:2
Heun M (1992) Mapping quantitative powdery mildew resistance of barley using a restriction fragment length polymorphism map. Genome 35:1019–1025
Heun M, Kennedy AE, Anderson JA, Eapitan NLV, Sorrells ME, Tanksley SD (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34:437–447
Houston BR, Oswald JW (1948) Methods of inoculation of barley with the stripe disease, Helminthosporium gramineum (Abstr). Phytopathology 38:915
Jung M, Weldekidan T, Schaff D, Paterson A, Tingey S, Hawk J (1994) Generation-means analysis and quantitative trait locus mapping of anthracnose stalk rot genes in maize. Theor Appl Genet 89:413–418
Knott SA, Haley CS (1992) Aspects of maximum likelihood methods for the mapping of quantitative trait loci in line crosses. Genet Res 60:139–151
Knudsen JCN (1981) Resistance to Pyrenophora graminea in spring barley varieties subjected to natural inoculum. Barley Genet 5:498–502
Knudsen JCN (1986) Resistance to barley leaf stripe. Z Pflanzenzuecht 96:161–168
Kreike CM, Dekoning JRA, Vinke JH, van Ooijen JW, Stiekema WJ (1994) Quantitatively-inherited resistance to Globodera pallida is dominated by one major locus in Solanum spegazzinii. Theor Appl Genet 88:764–769
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 136:705
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Leonards-Schippers C, Gieffers W, Schäfer-Pregl R, Ritter E. Knapp SJ, Salamini F, Gebhardt C (1994) Quantitative resistance to Phytophthora infestans in potato: a case study for QTL mapping in an allogamous plant species. Genetics 137:67–77
Lincoln S, Daly M, Lander E (1992) Mapping genes controlling quantitative traits with MAPMAKER/QTL 1.1. Technical report, 2nd edn. Whitehead Institute Press, Cambridge, Mass.
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
Platenkamp R (1976) Investigations on the infection pathway of Drechslera graminea in germinating barley. R Vet Agric Univ Yearb 1976, Copenhagen, Denmark, pp 49/2-64
Porta-Puglia A, Delogu G, Vannacci G (1986) Pyrenophora graminea on winter barley seed: effect on disease incidence and yield losses. J Phytopathol 117:26–33
Robertson DS (1985) A possible technique for isolating genomic DNA for quantitative traits in plants. J Theor Biol 117:1–10
SAS Institute (1988) SAS/STAT user's guide, release 6.03 edn. SAS Institute, Cary, N.C.
Shin JS, Chao S, Corpuz L, Blake T (1990) A partial map of the barley genome incorporating restriction fragment length polymorphism, polymerase chain reaction, isozyme and morphological marker loci. Genome 33:803–810
Skou JP, Haahr V (1987) Screening for and inheritance of resistance to barley leaf stripe (Drechslera graminea). Risø-R-554
Skou JP, Nielsen BJ, Haahr V (1994) Evaluation and importance of genetic resistance to leaf stripe in Western European barleys. Acta Agric Scand Sect B Soil Plant Sci 44:98–106
Smedegaard-Petersen V, Jørgensen J (1982) Resistance to barley leaf stripe caused by Pyrenophora graminea. Phytopathol Z 105:183–191
Tekauz A (1983) Reaction of Canadian barley cultivars to Pyrenophora graminea, the incitant of leaf stripe. Can J Plant Pathol 5:294–301
Wang G-L, Mackill DJ, Bornman JM, McCouch SR, Champoux MC, Nelson RJ (1994) RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. Genetics 136:1421–1434
Author information
Authors and Affiliations
Additional information
Communicated by F. Salamini
Rights and permissions
About this article
Cite this article
Pecchioni, N., Faccioli, P., Toubia-Rahme, H. et al. Quantitative resistance to barley leaf stripe (Pyrenophora graminea) is dominated by one major locus. Theoret. Appl. Genetics 93, 97–101 (1996). https://doi.org/10.1007/BF00225733
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225733