Abstract
Resistance to Plasmodiophora brassicae Woron, the causal fungus of clubroot, was examined in an F2 population of a cross between a clubroot-resistant kale (Brassica oleracea L. var. acephala) and a susceptible cauliflower (Brassica oleracea L. var. botrytis). QTL detection was performed with RAPD markers. Two resistance notations, carried out at different times after inoculation, were used. Three markers were associated with these two notations and three were specifically linked to only one notation. QTL analysis suggests the existence of at least two genetic mechanisms implicated in the resistance phenomenon.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adam-Blondon AF, Sévignac M, Dron M (1994) A genetic map of common bean to localize specific resistance genes anthracnose. Genome 37:915–924
Buczacki ST (1983) Plasmodiophora. An inter-relationship between biological and practical problems. In: Buczaki S.T. (ed) Zoosporic plant pathogens — a modern perspective Academic press, London, pp 161–191
Buczacki ST, Toxopeus H, Mattusch P, Johnston TD, Dixon GR, Hobolth LA (1975). Study of physiological specialization in Plasmodiophora brassicae:proposals for attempted rationalization through an international approach. Trans Br Mycol Soc 65:295–303
Butcher DN, El-Tigani, Ingram DS (1974) The role of the indole glucosinolates in the clubroot disease of the Cruciferae. Physiol Plant Pathol 4:127–141
Crête R, Chiang MS (1980) Screening Brassicas for resistance to clubroot, Plasmodiophora brassicae Wor. Can Plant Dis Surv, 60:17–19
Crisp P, Crute IR, Sutherland RA, Angell SM, Bloor K, burgess H, Gordon PL (1989) The exploitation of genetic ressources of Brassica oleracea in breeding for resistance to clubroot (Plasmodiophora brassicae). Euphytica 42:215–226
Crute IR, Phelps K, Barnes A, Buczacki ST, Crisp P (1983) The relationship between genotypes of three Brassica species and collections of Plasmodiophora brassicae. Plant Pathol 32:405–420
Dekhuijzen HM (1979). Electron microscopic studies on the root hairs and cortex of a susceptible and a resistant variety of Brassica campestris infected with Plasmodiophora brassicae. Neth J Pathol 85:1–17
Dias JS, Ferreira ME, Williams PH (1993) Screening of Portuguese cole landraces (Brassica oleracea L.) with Peronospora parasitica and Plasmodiophora brassicae. Euphytica 67:135–141
Dixon GR, Robinson DL (1986) The susceptibility of Brassica oleracea cultivars to Plasmodiophora brassicae (clubroot). Plant Pathol 35:101–107
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Figdore SS, Ferreira ME, Slocum MK, Williams PH (1993) Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea L. Euphytica 69:33–44
Grandclément C (1995) Analyse de la résistance à la hernie des Crucifères (Plasmodiophora brassicae Wor.) dans l'espèce Brassica oleracea L. Approche génétique et marquage moléculaire. PhD thesis, ENSA, Rennes, France
Grandclément C, Tomas G (1996) Genetic analysis of resistance to clubroot (Plasmodiophora brassicae Wor.) in two Brassica oleracea groups (ssp. acephala and ssp. botrytis) through diallel analysis. Plant Breed vol 115 (in press)
Ingram DS, Tommerup IC (1972) The life history of Plasmodiophora brassicae Woron. Proc R Soc Lond Ser B Biol Sci 180: 103–112
Landry BS, Hubert N, Crête R, Chang MS, Lincoln SE, Etoh T (1992) A genetic map of Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin). Genome 35:409–419
Laurens F (1991) Etude du déterminisme génétique de la résistance à la hernie des Crucifères (Plasmodiophora brassicae Woron) dans l'espèce Brassica oleracea. PhD thesis, Université de Rennes I, France
Laurens F, Thomas G (1993) Inheritance of resistance to clubroot (Plasmodiophora brassicae Wor.) in Kale (Brassica oleracea ssp. acephala). Hereditas 119:253–262
Lebfevre V, Chèvre AM (1995) Tools for making plant disease and pest resistance genes:a review. Agronomie 15:3–19
Michelmore R (1995) Molecular approaches to manipulation of disease resistance genes. Annu Rev Phytopathol 15:393–427
Monteiro AA, Williams PH (1989) The exploration of resources of Portuguese cabbage and Kale for resistance to several Brassica diseases. Euphytica 41:215–225
Naiki T, Kageyama K, Ikegami H (1978) The relation of spore density of Plasmodiophora brassicae Wor. to the root hair infection and club formation in Chinese cabbage. Ann Phytopathol Soc Japan 44:432–439
Pink DAC, King GJ, Ockendon DJ, Mclenaghan R, Lynn J (1994) Use of anther culture and molecular markers in breeding for resistance to Plasmodiophora brassicae. In:ISHS Symp on Brassicas, 9th Crucifer Genet Wkshp, Lisbon, p 15
Rouxel F, Lejeune B, Sanson MT (1991) Etat actuel des connaissances sur la hernie du chou-fleur en Bretagne. Aujourd'hui et Demain 30:5–14
Voorrips RE (1992) Root hair infection by Plasmodiophora brassicae in clubroot-resistant and -susceptible genotypes of Brassica oleracea, B. rapa and B. napus. Neth PI Pathol 98:361–368
Weisaeth G (1974) Some problems and results in breeding for clubroot resistance in cole crops. Proc Cruciferae Eucarpia Meeting, pp 101-107
Williams PH, Aist SJ, Aist JR (1971) Response of cabbage root hairs to infection by Plasmodiophora brassicae. Can J Bot, 49: 41–47
Young ND, Danesh D, Menancio-Hautea D, Kumar L (1993) Mapping oligogenic resistance to powdery mildew in mungbean with RFLPs. Theor Appl Genet 87:243–249
Author information
Authors and Affiliations
Additional information
Communicated by H. F. Linskens
Rights and permissions
About this article
Cite this article
Grandclément, C., Thomas, G. Detection and analysis of QTLs based on RAPD markers for polygenic resistance to Plasmodiophora brassicae Woron in Brassica oleracea L.. Theoret. Appl. Genetics 93, 86–90 (1996). https://doi.org/10.1007/BF00225731
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225731