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Comparative cellular localization of corticotropin and melanotropin in lerot adenohypophysis (Eliomys quercinus)

An immunohistochemical study

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Summary

Corticotropin and melanotropin producing cells were localized in the adenohypophysis of normal Lerots by using antibodies against synthetic corticotropins (anti β1–24 ACTH, anti α17–39 ACTH, anti α25–39 ACTH), and melanotropins (anti αMSH, anti βMSH). All the anticorticotropin sera stained the same cells both in the anterior lobe and in the intermediate lobe. The anti αMSH serum only stained a few cells, exclusively located in the intermediate lobe. These αMSH cells were not stained with anticorticotropin antibodies. The anti βMSH serum revealed all the cells stained with anticorticotropin and anti αMSH sera. Absorption tests showed that the 4–10 heptapeptide common to ACTH and MSH, is not responsible for the immunohistochemical staining. The staining of only some corticotrophs with the anti 4–10 ACTH serum might indicate the presence in these cells of a peptide with an accessible 4–10 site. These results are discussed

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Authors and Affiliations

  1. Laboratory of Histology of the Faculty of Medicine, Lille, France

    G. Tramu & M. P. Dubois

  2. Laboratory of the Physiology of Reproduction, INRA, CNRZ, Nouzilly, France

    G. Tramu & M. P. Dubois

Authors
  1. G. Tramu
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  2. M. P. Dubois
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Additional information

We thank A. Pillez for technical assistance (C.N.R.S.). This work was supported by a grant from U.E.R. III Lille 1976

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Tramu, G., Dubois, M.P. Comparative cellular localization of corticotropin and melanotropin in lerot adenohypophysis (Eliomys quercinus). Cell Tissue Res. 183, 457–469 (1977). https://doi.org/10.1007/BF00225660

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