Summary
Phlebotomy of untreated and iron-loaded rats results in a significant decrease in total liver iron. In ironloaded rats a marked decrease in iron-containing particles is observed ultrastructurally in lysosomes and cytoplasm of hepatic sinusoidal cells but not in parenchymal cells. This remarkable phenomenon was further investigated in a morphometric study, based on element-specific (iron) distribution images made in situ in the parenchymal cell by means of electron energy loss spectroscopy. With the use of this technique it could be shown that in spite of phlebotomy the ferritin iron content of the iron-loaded liver parenchymal cell is not decreased.
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Andrews SC, Brady MC, Treffry A, Williams JM, Mann S, Cleton MI, Bruijn WC de, Harrison PM (1988) Studies on haemosiderin and ferritin from iron-loaded rat liver. Biol Metals 1:33–42
Bauer R (1987) Zeiss Information. MEM Oberkochen 5:10–15
Berkel ThJC van, Dekker CJ, Kruijt JK, Eijk HG van (1987) The interaction in vivo of transferrin and asialotransferrin with liver cells. Biochem J 243:715–722
Clegg CA, Fitton JE, Harrison PM, Treffry A (1980) Ferritin molecular structure and iron-storage mechanisms. Prog Biophys Molec Biol 36:53–86
Cleton MI, Bruijn WC de, Jong AWW de, Jeu-Jaspars CMH, Sorber LWJ (1988) A study with E.E.L.S. on ferritin. In: Proceedings of the 29th Dutch Federation Meeting, Utrecht 6–7 April. Published by the Dutch Foundation Federation of Medical Scientific Societies. A 70
Dekker CJ, Kroos MJ, Heul C van der, Eijk HG van (1985) Uptake of sialo and asialo transferrins by isolated rat hepatocytes. Comparison of a heterologous and a homologous system. Int J Biochem 17:701–706
Düllman J, Wulfhekel U, Hausmann K (1978) Differences between Kupffer cells and endothelial cells of human liver in iron storage and release under normal conditions, after i.v. iron administration and in iron overload. In: Wisse E, Knook DL (eds) Kupffer cells and other sinusoidal liver Cells. Elsevier Biomedical Press, Amsterdam, pp 233–241
Farrant JL (1954) An electron microscopic study of ferritin. Biochem Biophys Acta 13:569–575
Ghadially FN, Schneider RJ, Lalonde J-MA (1981) Haemosiderin deposits in the human cornea. J Submicrosc Cytol 13:455–460
Harrison PM (1977) Ferritin: an iron-storage molecule. Semin Hematol 14:55–70
Haurani FI, Meyer A, O'Brien R (1973) Production of transferrin by the macrophage. Res J Reticuloendothel Soc 14:309–316
Hezel UB, Bauer R (1987) Zeiss Information. MEM Oberkochen 5:28–30
Hoy TG, Jacobs A (1983) Ferritin polymers and the formation of haemosiderin. Br J Haematol 48:593–602
Hultcrantz R, Glaumann H (1982) Studies on the rat liver folllowing iron overload. Biochemical studies following iron mobilization. Lab Invest 46:383–393
Hultcrantz R, Arborgh B, Wroblweski R, Ericsson JLE (1979) Studies on the rat liver following iron overload. Electron probe X-ray microanalysis of acid phosphatase and iron. Am J Pathol 96:625–640
Hultcrantz R, Arborgh B, Wroblewski R, Ericsson JLE (1980) Studies on the rat liver following iron overload. Acta Pathol Microbiol Scand Sect A 88:341–353
Mack U, Storey EL, Powell LW, Halliday JW (1985) Characterization of the binding of ferritin to the rat hepatic ferritin receptor. In: Spik G, Montreuil J, Crichton RR, Mazurier J (eds) Proteins of iron storage and transport. Elsevier Science Publishers, Amsterdam New York Oxford, pp 203–206
Malis T, Cheng SC, Egerton RF (1988) EELS log-ratio technique for specimen-thickness measurement in the TEM. J Electron Microsc Tech 8:193–200
Mostert LJ, Cleton MI, Bruijn WC de, Koster JF, Eijk HG van (1989) Studies on ferritin in rat liver and spleen during repeated phlebotomy. Int J Biochem 21:39–47
Ottensmeyer FP, Andrew JW (1980) High-resolution microanalysis of biological specimens by electron energy loss spectroscopy and by electron spectroscopic imaging. J Ultrastruct Res 72:336–341
Richter GW (1957) A study of haemosiderosis with the aid of electron microscopy: With observations on the relationship between haemosiderin and ferritin. J Exp Med 106:203–218
Richter GW (1984) Studies of Iron overload. Lab Invest 50:26
Trinder D, Morgan E, Baker E (1986) The mechanisms of iron uptake by fetal rat hepatocytes in culture. Hepatology 6:852–858
Williams MA (1977) Practical Methods in Electron Microscopy vol. 6. In: Glauert AM (ed) Quantitative methods in Biology. North-Holland Publishing Company Amsterdam New York Oxford. Chapter 2: Stereological techniques, pp 5–84
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Cleton, M.I., Mostert, L.J., Sorber, L.W.J. et al. Effect of phlebotomy on the ferritin iron content in the rat liver as determined morphometrically with the use of electron energy loss spectroscopy. Cell Tissue Res. 256, 601–605 (1989). https://doi.org/10.1007/BF00225610
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DOI: https://doi.org/10.1007/BF00225610