Summary
The objective of this in-vitro study was to examine whether the diencephalic floor or the mesenchyme is involved in differentiation of LH cells in the developing rat adenohypophysis. Overall growth of the adenohypophysial tissue was retarded when the adenohypophysial primordium was cultivated after enzymatic removal of the diencephalic floor on days 11.5 and 12.5 of gestation. This malgrowth was more marked when the brain was separated on day 11.5; most expiants retained a simple cystiform structure that consisted of a few layers of undifferentiated cells. Removal of the brain also caused a highly significant decrease (P < 0.001) in the number of immunoreactive LH cells, if it was performed on day 11.5 but not day 12.5. Mesenchyme had little effect on the adenohypophysial growth or the number of immunopositive cells. Cultivation of the adenohypophysial primordium with the diencephalic floor resulted in the appearance of many immunoreactive LH cells. The number of LH cells significantly decreased, however, when the co-cultivated brain completely surrounded the adenohypophysial tissue.
These results indicate that in 11.5-day-old fetal rats the diencephalic floor is indispensable for the initial proliferation of adenohypophysial primordial cells and for the early determinating process of LH cells. Once determined, the development of LH cells may proceed without the surrounding tissues. The cytodifferentiation seems to be rather inhibited when in contact with the brain. The significance of the intimate spatial relationship between developing LH cells and the surrounding mesenchyme is also discussed.
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References
Atwell WJ (1935) Differentiation and function of heterotopic autoplastic transplants of the amphibian hypophysis. Proc Soc Exp Biol Med 33:224–226
Atwell WJ (1937) Functional transplants of the primordium of the epithelial hypophysis in amphibia. Anat Rec 68:431–447
Atwell WJ, Taft JW (1940) Functional transplants of epithelial hypophysis in three species of Amblystoma. Proc Soc Exp Biol Med 44:53–55
Begeot M, Dupouy JP, Dubois MP, Dubois PM (1981) Immunocytological determination of gonadotropic and thyrotropic cells in fetal rat anterior pituitary during normal development and under experimental conditions. Neuroendocrinology 32:285–294
Begeot M, Dubois MP, Dubois PM (1982) Comparative study in vivo and in vitro of the differentiation of immunoreactive corticotropic cells in fetal rat anterior pituitary. Neuroendocrinology 35:255–264
Begeot M, Hemming FJ, Martinat N, Dubois MP, Dubois PM (1983a) Gonadotropin releasing hormone (GnRH) stimulates immunoreactive lactotrope differentiation. Endocrinology 112:2224–2226
Begeot M, Dubois MP, Dubois PM (1983b) Comparative study in vivo and in vitro of the differentiation of immunoreactive gonadotropic cells in fetal rat anterior pituitary. Neuroendocrinology 37:52–58
Begeot M, Morel G, Rivest RW, Aubert ML, Dubois MP, Dubois PM (1984) Influence of gonadoliberin on the differentiation of rat gonadotrophs: an in vivo and in vitro study. Neuroendocrinology 38:217–225
Blount RF (1932) Transplantation and extirpation of the pituitary rudiment and the effects upon pigmentation in the urodele embryo. J Exp Zool 63:113–142
Chang CY (1957) Hypothalectomy in Rana pipiens neurulae. Anat Rec 128:531–532
Driskoll WT, Eakin RM (1955) The effects of sucrose on amphibian development with special reference to the pituitary body. J Exp Zool 129:149–176
Etkin W (1935) Effects of multiple pituitary primordia in the tadpole. Proc Soc Exp Biol Med 32:1653–1655
Etkin W (1958a) Independent differentiation in components of the pituitary complex in the wood frog. Proc Soc Exp Biol Med 97:388–393
Etkin W (1958b) Embryonic determination of the adenohypophysis in the wood frog, R. sylvatica. Anat Rec 131:548
Ferrand R (1969) Influence inductrice exercée par le plancher encéphalique sur l'ébauche adénohypophysaire aux jeunes stades du développement de l'embryon de poulet. C R Acad Sci (Paris) 268:550–553
Ferrand R (1972) Etude expérimentale de facteurs de la différenciation cytologique de l'adénohypophyse chez l'embryon de poulet. Arch Biol (Liège) 83:297–371
Ferrand R, Le Douarin N (1968) Différenciation de tissue adénohypophysaire à partir de la poche de Rathke prélevée après le stade de la détermination chez l'embryon de poulet et mise au contact de divers mésenchymes. C R Soc Biol 162:2215–2218
Ferrand R, Nanot J (1968) Différenciation de la poche de Rathke isolée de l'ébauche nerveuse de l'hypophyse et associée à un mésenchyme hétérologue chez la souris. C R Soc Biol 162:983–986
Gash D, Ahmad N, Weiner R, Schechter J (1980) Development of presumptive mammotrophs in isografts is dependent on the endocrine state of the host. Endocrinology 106:1246–1252
Gross DS, Baker BL (1979) Developmental correlation between hypothalamic gonadotropin-releasing hormone and hypophysial luteinizing hormone. Am J Anat 154:1–10
Hanaoka Y (1967) The effects of posterior hypothalectomy upon the growth and metamorphosis of the tadpole of Rana pipiens. Gen Comp Endocrinol 8:417–431
Kusakabe M, Sakakura T, Sano M, Nishizuka Y (1984) Early development of mouse anterior pituitary: role of mesenchyme. Dev Growth Differ 26:263–271
Le Douarin G, Ferrand R (1968) Différenciation fonctionelle de l'ébauche épithéliale de l'adénohypophyse isolée du plancher encéphalique: activité thyréotrope. C R Acad Sci (Paris) 266:697–699
Le Douarin N, Ferrand R, Le Douarin G (1967) La différenciation de l'ébauche épithéliale de l'hypophyse séparée du plancher encéphalique et placée dans des mésenchymes hétérologues. C R Acad Sci (Paris) 264:3027–3029
Lieberman ME, Slabaugh MB, Rutledge JJ, Gorski J (1983) The role of estrogen in the differentiation of prolactin producing cells. J Steroid Biochem 19:275–281
Nemeskéry A, Németh A, Sétáló G, Vigh S, Halász B (1976) Cell differentiation of the fetal rat anterior pituitary in vitro. Cell Tissue Res 170:263–273
Sétáló G, Nakane PK (1976) Functional differentiation of the fetal anterior pituitary cells in the rat. Endocrinol Exp 10:155–166
Sobel H (1958) The behaviour in vitro of dissociated embryonic pituitary tissue. J Embryol Exp Morphol 6:518–526
Sternberger LH, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Watanabe YG (1982a) Effects of brain and mesenchyme upon the cytogenesis of rat adenohypophysis in vitro. I. Differentiation of adrenocorticotropes. Cell Tissue Res 227:257–266
Watanabe YG (1982b) An organ culture study on the site of determination of ACTH and LH cells in the rat adenohypophysis. Cell Tissue Res 227:267–275
Watanabe YG, Daikoku S (1976) Immunohistochemical study on adenohypophysial primordia in organ culture. Cell Tissue Res 166:407–412
Watanabe YG, Daikoku (1979) An immunohistochemical study on the cytogenesis of adenohypophysial cells in fetal rats. Dev Biol 68:557–567
Watanabe YG, Matsumura H, Daikoku S (1973) Electron microscopic study of rat pituitary primordium in organ culture. Z Zellforsch 146:453–461
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Watanabe, Y.G. Effects of brain and mesenchyme upon the cytogenesis of rat adenohypophysis in vitro. Cell Tissue Res. 242, 49–55 (1985). https://doi.org/10.1007/BF00225562
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DOI: https://doi.org/10.1007/BF00225562