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Changes in a photoreceptor polypeptide correlating with an early-onset retinal dystrophy in the cat

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Abstract

A preparation of rod outer segments has been used to study the polypeptides characteristic of an early-onset retinal dystrophy in cats (Rdy) by two-dimensional gel electrophoresis. Comparison of 2-D gels of rod outer segment preparations from retinas of normal and Rdy animals shows several differences. In particular, a polypeptide of Mr 51 kDa and pI 7.5 is present at increased levels in preparations from Rdy cats at 6 weeks, 9 weeks and 12.5 weeks of age but not at 3.5 weeks.

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Abbreviations

cGMP:

Guanosine 3′

5′:

Cyclic Monophosphate

SDS-PAGE:

Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis

TRIS:

2-amino-2(hydroxymethyl)-1,3 propandiol

PMSF:

Phenylmethylsulphonylfluoride

IEF:

Isoelectrofocussing

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Authors and Affiliations

  1. Department of Immunogenetics, Animal Health Trust, PO Box 5, CB8 7DW, Newmarket, Suffolk, UK

    Nigel G. Holmes & Roger Curtis

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  1. Nigel G. Holmes
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  2. Roger Curtis
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Holmes, N.G., Curtis, R. Changes in a photoreceptor polypeptide correlating with an early-onset retinal dystrophy in the cat. Mol Cell Biochem 107, 111–117 (1991). https://doi.org/10.1007/BF00225514

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  • DOI: https://doi.org/10.1007/BF00225514

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