Abstract
The level of polymorphism using genomic and cDNA probes with a number of restriction enzymes and the inheritance of the RFLP loci was investigated in E. nitens. The polymorphism detected with 366 genomic and cDNA probes and three to six restriction enzymes was analysed in three-generation outbred pedigrees. No difference in the level of polymorphism detected with genomic versus cDNA probes was observed. There was a difference in the efficiency of detection of polymorphism with six different restriction enzymes, with three of the enzymes (BglII, DraI and EcoRI) showing substantially more polymorphism than the others. There was no significant correlation between the size of the DNA fragments generated by the enzymes and the detection of polymorphism. Several cases of restriction-site mutations resulting in a polymorphism were observed. The inheritance of 69 loci was analysed in two pedigrees resulting from interpopulational crosses. The majority of the loci segregated according to expected ratios with distortion observed in only 3% of loci. Probes from the cDNA library detected a greater proportion of loci with more than two alleles than did probes from the genomic library. The high polymorphism, large number of alleles, and ease of interpretation of RFLPs in E. nitens means that they will be useful in a range of applications such as genetic linkage maps and paternity analysis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Apuya NR, Frazier BL, Keim P, Roth EJ, Lark KG (1988) Restriction fragment length polymorphisms as genetic markers in soybean, Glycine max (L.) merrill. Theor Appl Genet 75:889–901
Brummer EC, Kochert G, Bouton JH (1991) RFLP variation in diploid and tetraploid alfalfa. Theor Appl Genet 83:89–96
Burr B, Burr FA, Thompson KH, Albertson MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526
Byrne M, Moran GF (1994) Population divergence in the chloroplast genome of Eucalyptus nitens. Heredity 73:18–28
Byrne M, Moran GF, Tibbits WN (1993) Restriction map and maternal inheritance of chloroplast DNA in Eucalyptus nitens. J Hered 84:218–220
Devey ME, Jermstad KD, Tauer CG, Neale DB (1991) Inheritance of RFLP loci in a loblolly pine three-generation pedigree. Theor Appl Genet 83:238–242
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Annal Biochem 132:6–13
Figdore SS, Kennard WC, Song KM, Slocum MK, Osborn TC (1988) Assessment of the degree of restriction fragment length polymorphism in Brassica. Theor Appl Genet 75:833–840
Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78:65–75
Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F (1991) RFLP maps of potato and their alignment with the homeologous tomato genome. Theor Appl Genet 83:49–57
Grattapaglia D, Bradshaw H (1994) Nuclear DNA content of commercially important Eucalyptus species and hybrids. Can J For Res (in press)
Havey MJ, Muehlbauer FJ (1989) Linkages between restriction fragment length, isozyme and morphological markers in lentil. Theor Appl Genet 77:395–401
Helentjaris T, King G, Slocum M, Siedenstrang C, Wegman S (1985) Restriction fragment length polymorphisms as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol Biol 5:109–118
Helentjaris T, Weber D, Wright S (1988) Identification of the genomic locations of duplicate nucleotide sequences in maize by analysis of restriction fragment length polymorphisms. Genetics 118:353–363
Kianian SF, Quiros CF (1992) Generation of a Brassica oleracea- composite RFLP map: linkage arrangements among various populations and evolutionary implications. Theor Appl Genet 84:544–554
Landry BS, Kesseli RV, Farrara B, Michelmore RW (1987a) A genetic linkage map of lettuce (Lactuca sativa L.) with restriction fragment length polymorphism, isozyme, disease resistance and morphological markers. Genetics 116:331–337
Landry BS, Kesseli R, Leung H, Michelmore RW (1987b) Comparison of restriction endonucleases and sources of probes for their efficiency in detecting restriction fragment length polymorphisms in lettuce (Lactuca sativa L.). Theor Appl Genet 74:646–653
Liu Z, Furnier GR (1993) Inheritance and linkage of allozymes and restriction fragment length polymorphisms in trembling aspen. J Hered 84:419–424
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Miller JC, Tanksley SD (1990) Effect of different restriction enzymes, probe source and probe length on detecting restriction fragment length polymorphism in tomato. Theor Appl Genet 80:385–389
Moran GF (1992) Patterns of genetic diversity in Australian tree species. New Forests 6:49–66
Moran GF, Bell JC (1983) Eucalyptus. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding. Part B. Elsevier Science Publishers, Amsterdam, pp 423–441
Song KM, Suzuki IY, Slocum MK, Williams PH, Osborn TC (1991) A linkage map of Brassica rapa (syn. campestris) based on restriction fragment length polymorphism loci. Theor Appl Genet 82:296–304
Wang ZY, Tanksley SD (1989) Restriction fragment length polymorphism in Oryza sativa L. Genome 32:1113–1118
Author information
Authors and Affiliations
Additional information
Communicated by P. M. A. Tigerstedt
Rights and permissions
About this article
Cite this article
Byrne, M., Moran, G.F., Murrell, J.C. et al. Detection and inheritance of RFLPs in Eucalyptus nitens . Theoret. Appl. Genetics 89, 397–402 (1994). https://doi.org/10.1007/BF00225372
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225372