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Purification and characterization of cytoplasmic 14C-arginine rich basic proteins of Ehrlich ascites tumor cells

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Summary

Arginine rich basic proteins from cytoplasm of Ehrlich ascites tumor cells have been separated and partially characterized. All proteins show a cationic character with the following isoelectric points: 8.45, 8.60, 8.70, 8.90, and possess various amount of arginine. The protein of the highest molecular weight (75000) has the greatest amount of arginine (18.1%), specific radioactivity (19760 cpm/mg, min) and isoelectric point (8.9). The significance of those proteins in the cytoplasm of tumor cells has been discussed briefly.

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References

  1. Farbiszewski, R. and Rzeczycki, W., 1974. Neoplasma 21, 363–366.

    Google Scholar 

  2. Farbiszewski, R., Wincewicz, A. and Rzeczycki, W., 1977. Mol. Cell. Biochem. 17, 3–6.

    Google Scholar 

  3. Farbiszewski, R., Kilczewska, D. and Rzeczycki, W., 1980. Experientia 36, 125–126.

    Google Scholar 

  4. Farbiszewski, R. and Rzeczycki, W., 1973. Neoplasma 20, 217–219.

    Google Scholar 

  5. Farbiszewski, R., Worowski, K. and Rzeczycki, W., 1975. Bull. Polon. Acad. Sci., ser. sci. biol. 22, 829–836.

    Google Scholar 

  6. Wincewicz, A., Farbiszewski, R., Rzeczycki, W. and Tomasiak, M., 1979. Bull. Acad. Polon. Sci., ser. sci. biol. 27, 93–98.

    Google Scholar 

  7. Farbiszewski, R., Wincewicz, A., Bańkowski, E. and Gabryel, H., 1979. Biochem. Biophys. Res. Commun. 86, 1096–1101.

    Google Scholar 

  8. Farbiszewski, R., Rzeczycki, W., Worowski, K. and Glowiński, S., 1973. Neoplasma 20, 203–208.

    Google Scholar 

  9. Panyim, S. and Chalkley, R., 1969. Arch. Biochem. Biophys. 130, 337–346.

    Google Scholar 

  10. Lowry, O. H., Rosenbrough, N. J., Farr, A. L. and Randal, R. J., 1951. J. Biol. Chem. 193, 265–275.

    CAS  PubMed  Google Scholar 

  11. Parish, C. R. and Marchalonis, J. J., 1970. Anal. Biochem. 34, 436–450.

    Google Scholar 

  12. Vesteberg, O. and Svensson, H., 1966. Acta Chem. Scand. 20, 820–834.

    Google Scholar 

  13. Farbiszewski, R., Worowski, K. and Rzeczycki, W., 1972. Chem. Anal. 17, 133–137.

    Google Scholar 

  14. Efimow, M. L., Jakowlewa, S. S. and Ismailow, B. I., 1969. Wop. Onkol. 15, 94–96.

    Google Scholar 

  15. Me Cune, S., Morris, H. P. and Weber, G., 1979. Biochem. Biophys. Acta 561, 410–420.

    Google Scholar 

  16. Ceccarini, C. and Maggio, R., 1969. Biochim. Biophys. Acta 190, 556–559.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Inorganic and Analytic Chemistry, Institute of Chemistry and Biophysics, Medical School, Bialystok 8, Poland

    Ryszard Farbiszewski & Danuta Kilczewska

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  1. Ryszard Farbiszewski
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  2. Danuta Kilczewska
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Farbiszewski, R., Kilczewska, D. Purification and characterization of cytoplasmic 14C-arginine rich basic proteins of Ehrlich ascites tumor cells. Mol Cell Biochem 33, 145–148 (1980). https://doi.org/10.1007/BF00225286

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  • DOI: https://doi.org/10.1007/BF00225286

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